Renilla Luciferase Recombinant Rabbit Monoclonal Antibody [SR07-830]
Catalog# ET1602-45
Renilla Luciferase Recombinant Rabbit Monoclonal Antibody [SR07-830]
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WB
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Sea pansy
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unconjugated
Overview
Product Name
Renilla Luciferase Recombinant Rabbit Monoclonal Antibody [SR07-830]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Sea pansy Renilla Luciferase aa 1-96 / 311.
Species Reactivity
Sea pansy
Validated Applications
WB
Molecular Weight
Predicted band size: 36 kDa
Positive Control
Luciferase recombinant protein.
Conjugation
unconjugated
Clone Number
SR07-830
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500-1:2,000
Target
Function
Upon binding the substrate, the enzyme catalyzes an oxygenation, producing a very short-lived hydroperoxide that cyclizes into a dioxetanone structure, which collapses, releasing a CO2 molecule. The spontaneous breakdown of the dioxetanone releases the energy (about 50 kcal/mole) that is necessary to generate the excited state of the coelenteramide product, which is the singlet form of the monoanion. In vivo the product undergoes the process of nonradiative energy transfer to an accessory protein, a green fluorescent protein (GFP), which results in green bioluminescence. In vitro, in the absence of GFP, the product emits blue light.
Background References
1. Shen L et al. Safe and Sensitive Antiviral Screening Platform Based on Recombinant Human Coronavirus OC43 Expressing the Luciferase Reporter Gene. Antimicrob Agents Chemother 60:5492-503 (2016).
2. Loening AM et al. Crystal structures of the luciferase and green fluorescent protein from Renilla reniformis. J Mol Biol. 374(4):1017-28 (2007).
Synonyms
Coelenterazine h 2-monooxygenase
Renilla luciferin 2 monooxygenase antibody
Renilla type luciferase antibody
Images
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Western blot analysis of Renilla Luciferase on luciferase recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1602-45, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"