Phospho-V-Myb+C-Myb (S11) Recombinant Rabbit Monoclonal Antibody [SZ04-81]
Catalog# ET1603-41
Phospho-V-Myb+C-Myb (S11) Recombinant Rabbit Monoclonal Antibody [SZ04-81]
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IF-Cell
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IF-Tissue
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IHC-P
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Human
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unconjugated
Overview
Product Name
Phospho-V-Myb+C-Myb (S11) Recombinant Rabbit Monoclonal Antibody [SZ04-81]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding Ser11 of Human V-Myb+C-Myb.
Species Reactivity
Human
Validated Applications
IF-Cell, IF-Tissue, IHC-P
Molecular Weight
Predicted band size: 72 kDa
Positive Control
AGS, human tonsil tissue, MCF-7.
Conjugation
unconjugated
Clone Number
SZ04-81
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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IF-Cell
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1:100
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IF-Tissue
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1:50-1:200
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IHC-P
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1:200-1:1,000
Target
Function
The Myb family of transcription factors regulates proliferation, differentiation and apoptosis of hematopoletic cells. The avian myeloblastosis viral (v-Myb) protein is nuclear and binds to specific DNA sequences. The gene encoding for v-Myb is oncogenic, causing monoblastic leukemia and transforming myelomonocytic cells by deregulating the expression of specific target genes. v-Myb functions as a transcriptional activator, and it can repress biologically relevant genes such as Ets-2, which promotes macrophage differentiation. The proto-oncogene c-Myb encodes for a nuclear protein that plays a role in transcriptional regulation and may be essential for hematopoietic cell proliferation. A-Myb is also member of the Myb family that is expressed in proliferating B cell centroblasts. Transgenic mice overexpressing A-Myb posses enhanced hyperplasia of the lymph nodes.
Background References
1. Costa AF et al. Analysis of MYB oncogene in transformed adenoid cystic carcinomas reveals distinct pathways of tumor progression. Lab Invest 94:692-702 (2014).
2. Li L et al. Targeting poly(ADP-ribose) polymerase and the c-Myb-regulated DNA damage response pathway in castration-resistant prostate cancer. Sci Signal 7:ra47 (2014).
Post-translational Modification
Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.; Phosphorylated by NLK on multiple sites, which induces proteasomal degradation.; Phosphorylated by HIPK1. This phosphorylation reduces MYB transcription factor activity but not MYB protein levels.
Subcellular Location
Nucleus, cytosol.
UNIPROT
Synonyms
C myb antibody
C myb protein antibody
Cmyb antibody
MYB antibody
Myb proto oncogene antibody
V myb myeloblastosis viral oncogene homolog antibody
Images
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Immunocytochemistry analysis of AGS cells labeling Phospho-V-Myb+C-Myb (S11) with Rabbit anti-Phospho-V-Myb+C-Myb (S11) antibody (ET1603-41) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-V-Myb+C-Myb (S11) antibody (ET1603-41) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Phospho-V-Myb+C-Myb (S11) antibody (ET1603-41) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1603-41) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"