PBR Recombinant Rabbit Monoclonal Antibody [SA90-03]
Overview
Product Name
PBR Recombinant Rabbit Monoclonal Antibody [SA90-03]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human PBR aa 120-169 / 169.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell, IF-Tissue, IHC-P, IP, FC
Target Molecular Weight
Predicted band size: 19 kDa
Positive Control
HCT 116 cell lysate, THP-1 cell lysate, 293T cell lysate, SK-OV-3 cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, PC-12 cell lysates, THP-1, human colon tissue, human kidney tissue, mouse colon tissue, mouse kidney tissue, human colon carcinoma tissue, human prostate carcinoma tissue.
Conjugation
unconjugated
Clone Number
SA90-03
RRID
Reactivity Data
Verified (internally validated)
Reported in literature (not internally validated)
Predicted reactive (based on sequence homology)
Not recommended (failed internal validation)
| WB | IHC-P | FC | IF-Cell | IF-Tissue | IP | |
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| mouse |
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| rat |
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Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:2,000
-
IF-Cell
-
1:1,000
-
IF-Tissue
-
1:50-1:200
-
IHC-P
-
1:400-1:1,000
-
FC
-
1:1,000
-
IP
-
1-2μg/sample
Target
Function
Translocator protein (TSPO) is an 18 kDa protein mainly found on the outer mitochondrial membrane. It was first described as peripheral benzodiazepine receptor (PBR), a secondary binding site for diazepam, but subsequent research has found the receptor to be expressed throughout the body and brain. In humans, the translocator protein is encoded by the TSPO gene. It belongs to a family of tryptophan-rich sensory proteins. Regarding intramitochondrial cholesterol transport, TSPO has been proposed to interact with StAR (steroidogenic acute regulatory protein) to transport cholesterol into mitochondria, though evidence is mixed. In animals, TSPO (PBR) is a mitochondrial protein usually located in the outer mitochondrial membrane and characterised by its ability to bind a variety of benzodiazepine-like drugs, as well as to dicarboxylic tetrapyrrole intermediates of the haem biosynthetic pathway. TSPO has many proposed functions depending on the tissue. The most studied of these include roles in the immune response, steroid synthesis and apoptosis.
Background References
1. Tóth M et al. Acute neuroinflammation in a clinically relevant focal cortical ischemic stroke model in rat: longitudinal positron emission tomography and immunofluorescent tracking. Brain Struct Funct N/A:N/A (2015).
2. Banati RB et al. Positron emission tomography and functional characterization of a complete PBR/TSPO knockout. Nat Commun 5:5452 (2014).
Sequence Similarity
Belongs to the TspO/BZRP family.
Tissue Specificity
Found in many tissue types. Expressed at the highest levels under normal conditions in tissues that synthesize steroids.
Subcellular Location
Mitochondrion membrane
Synonyms
Benzodiazapine receptor (peripheral) antibody
Benzodiazepine peripheral binding site antibody
BPBS antibody
BZRP antibody
DBI antibody
IBP antibody
Isoquinoline carboxamide-binding protein antibody
MBR antibody
mDRC antibody
Mitochondrial benzodiazepine receptor antibody
ExpandImages
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Western blot analysis of PBR on different lysates with Rabbit anti-PBR antibody (ET1601-19) at 1/2,000 dilution.
Lane 1: HCT 116 cell lysate
Lane 2: THP-1 cell lysate
Lane 3: 293T cell lysate
Lane 4: SK-OV-3 cell lysate
Lane 5: NIH/3T3 cell lysate
Lane 6: RAW264.7 cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 19 kDa
Observed band size: 16 kDa
Exposure time: 1 minute 4 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-19) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of PBR on PC-12 cell lysates with Rabbit anti-PBR antibody (ET1601-19) at 1/2,000 dilution.
Lysates/proteins at 15 µg/Lane.
Predicted band size: 19 kDa
Observed band size: 16 kDa
Exposure time: 4 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-19) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
Western blot analysis of PBR on different lysates with Rabbit anti-PBR antibody (ET1601-19) at 1/2,000 dilution.
Lane 1: A549-si NT cell lysate
Lane 2: A549-si PBR cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 19 kDa
Observed band size: 16 kDa
Exposure time: 1 minute 51 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-19) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of THP-1 cells labeling PBR with Rabbit anti-PBR antibody (ET1601-19) at 1/1,000 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PBR antibody (ET1601-19) at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-PBR antibody (ET1601-19) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-19) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-PBR antibody (ET1601-19) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-19) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-PBR antibody (ET1601-19) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-19) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-PBR antibody (ET1601-19) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-19) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-PBR antibody (ET1601-19) at 1/400 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-19) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue with Rabbit anti-PBR antibody (ET1601-19) at 1/400 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-19) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Flow cytometric analysis of THP-1 cells labeling PBR.
Cells were fixed and permeabilized. Then stained with the primary antibody (ET1601-19, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). -
PBR was immunoprecipitated from 0.2 mg THP-1 cell lysate with ET1601-19 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using ET1601-19 at 1/1,000 dilution. Anti-Rabbit IgG for IP, AlpSdAbs® VHH(HRP) (025-100-005) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: THP-1 cell lysate (input)
Lane 2: ET1601-19 IP in THP-1 cell lysate
Lane 3: Rabbit IgG instead of ET1601-19 in THP-1 cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 1 seconds; ECL: K1801
Citation
-
Elucidating the mechanism of dibutyl phthalate on Alzheimer's disease through network toxicology, molecular docking and validation in mouse neuroblastoma cells
Journal: Food And Chemical Toxicology
DOI: 10.1016/j.fct.2025.115680
IF: 3.5
Application: WB
Reactivity: Mouse
Publish date: 2025 Aug
-
Developmental exposure to chlorpyrifos causes neuroinflammation via necroptosis in mouse hippocampus and human microglial cell line
Journal: Environmental Pollution
DOI:
IF: 8.9
Application: WB
Reactivity: Mouse
Publish date: 2022 Dec
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