Lane 1: Hela cell lysate
Lane 2: HepG2 cell lysate
Recombinant Rabbit monoclonal primary
Met(C-Met) Recombinant Rabbit Monoclonal Antibody [SJ19-05] (ET1606-45)
Synthetic peptide within c-terminal human met.
Hela cell lysate, HepG2 cell lysate, Hela, human tonsil tissue, human lung carcinoma tissue, human liver carcinoma tissue, human breast carcinoma tissue.
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Protein A affinity purified.
AUTS9 antibody; c met antibody; D249 antibody; Hepatocyte growth factor receptor antibody; HGF antibody; HGF receptor antibody; HGF/SF receptor antibody; HGFR antibody; MET antibody; Met proto oncogene tyrosine kinase antibody; MET proto oncogene, receptor tyrosine kinase antibody; Met proto-oncogene (hepatocyte growth factor receptor) antibody; Met proto-oncogene antibody; Met protooncogene antibody; MET_HUMAN antibody; Oncogene MET antibody; Par4 antibody; Proto-oncogene c-Met antibody; RCCP2 antibody; Scatter factor receptor antibody; SF receptor antibody; Tyrosine-protein kinase Met antibody
Belongs to the protein kinase superfamily. Tyr protein kinase family.
Expressed in normal hepatocytes as well as in epithelial cells lining the stomach, the small and the large intestine. Found also in basal keratinocytes of esophagus and skin. High levels are found in liver, gastrointestinal tract, thyroid and kidney. Also present in the brain. Expressed in metaphyseal bone (at protein level).
Autophosphorylated in response to ligand binding on Tyr-1234 and Tyr-1235 in the kinase domain leading to further phosphorylation of Tyr-1349 and Tyr-1356 in the C-terminal multifunctional docking site. Dephosphorylated by PTPRJ at Tyr-1349 and Tyr-1365. Dephosphorylated by PTPN1 and PTPN2.; Ubiquitinated. Ubiquitination by CBL regulates MET endocytosis, resulting in decreasing plasma membrane receptor abundance, and in endosomal degradation and/or recycling of internalized receptors.; (Microbial infection) Tyrosine phosphorylation is stimulated by L.monocytogenes InlB. Tyrosine phosphorylation is maximal 10-20 minutes after treatment with InlB and disappears by 60 minutes. The phosphorylated residues were not identified.
The c-Met oncogene was originally isolated from a chemical carcinogen-treated human osteogenic sarcoma cell line by transfection analysis in NIH/3T3 cells. The Met proto-oncogene product was identified as a transmembrane receptor-like protein with tyrosine kinase activity that is expressed in many tissues. A high proportion of spontaneous NIH/3T3 transformants overexpress c-Met and by transfection analysis the c-Met proto-oncogene has been shown to exhibit transforming activity. Tyrosine phosphorylation of apparently normal Met protein has also been observed in certain human gastric carcinoma cell lines . The c-Met gene product has been identified as the cell-surface receptor for hepatocyte growth factor, a plasminogen-like protein thought to be a humoral mediator of liver regeneration.