MEK1 Recombinant Rabbit Monoclonal Antibody [SZ22-01] (ET1603-20)
Catalog# ET1603-20
Rabbit Monoclonal to MEK1
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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IP
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FC
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Human
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Mouse
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Rat
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Cow
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Dog
Western blot analysis of MEK1 on different lysates with Rabbit anti-MEK1 antibody (ET1603-20) at 1/2,000 dilution.
Lane 1: HCT 116-si NT cell lysate
Lane 2: HCT 116-si MEK1 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 43 kDa
Observed band size: 45 kDa
Exposure time: 8 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1603-20) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
MEK1 Recombinant Rabbit Monoclonal Antibody [SZ22-01] (ET1603-20)
Immunogen
Synthetic peptide within n-terminal human mek1.
Host
Rabbit
Positive Control
HCT 116 cell lysate, HeLa cell lysate, HepG2 cell lysate, NIH/3T3 cell lysate, Mouse brain tissue lysate, Rat skeletal muscle tissue lysate, HeLa, NIH/3T3, human tonsil tissue, mouse pancreas tissue, rat pancreas tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SZ22-01
RRID
APPLICATION DILUTION
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WB
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1:1,000-1:2,000
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IF-Cell
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1:50-1:200
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IF-Tissue
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1:50-1:200
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IHC-P
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1:50-1:200
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FC
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1:100
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IP
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1-2μg/sample
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 43 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
MEK1
SYNONYMS
Dual specificity mitogen activated protein kinase kinase 1 antibody;Dual specificity mitogen-activated protein kinase kinase 1 antibody;ERK activator kinase 1 antibody;MAP kinase kinase 1 antibody;MAP kinase/Erk kinase 1 antibody;MAP2K1 antibody;MAPK/ERK kinase 1 antibody;MAPKK 1 antibody;MAPKK1 antibody;MEK 1 antibody;Mek1 antibody;MEKK1 antibody;Mitogen activated protein kinase kinase 1 antibody;MKK 1 antibody;MKK1 antibody;MP2K1_HUMAN antibody;PRKMK1 antibody;Protein kinase mitogen activated kinase 1 (MAP kinase kinase 1) antibody;Protein kinase mitogen activated, kinase 1 antibody
SEQUENCE SIMILARITIES
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
TISSUE SPECIFICITY
Widely expressed, with extremely low levels in brain.
POST-TRANSLATIONAL MODIFICATION
Phosphorylation at Ser-218 and Ser-222 by MAP kinase kinase kinases (BRAF or MEKK1) positively regulates kinase activity. Also phosphorylated at Thr-292 by MAPK1/ERK2 and at Ser-298 by PAK. MAPK1/ERK2 phosphorylation of Thr-292 occurs in response to cellular adhesion and leads to inhibition of Ser-298 phosphorylation by PAK. Autophosphorylated at Ser-218 and Ser-222, autophosphosphorylation is promoted by NEK10 following UV irradiation.; Acetylation by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
SUBCELLULAR LOCATION
Cytoplasm, Nucleus, Membrane, Cytoskeleton.
FUNCTION
Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Both MAP2K1/MEK1 and MAP2K2/MEK2 function specifically in the MAPK/ERK cascade, and catalyze the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2, leading to their activation and further transduction of the signal within the MAPK/ERK cascade. Activates BRAF in a KSR1 or KSR2-dependent manner; by binding to KSR1 or KSR2 releases the inhibitory intramolecular interaction between KSR1 or KSR2 protein kinase and N-terminal domains which promotes KSR1 or KSR2-BRAF dimerization and BRAF activation.
CITATIONS
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Cao H
The construction of intrahepatic cholangiocarcinoma model in zebrafish
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WB
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IHC
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zebrafish
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Citation
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Liu Yakun,et al
A rare germline BMP15 missense mutation causes hereditary ovarian immature teratoma in human
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IHC
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Mouse
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Citation
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PER3 plays anticancer roles in the oncogenesis and progression of breast cancer via regulating MEK/ERK signaling pathway
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WB
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Human
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Citation
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Identification of a Specific Phage as Growth Factor Alternative Promoting the Recruitment and Differentiation of MSCs in Bone Tissue Regeneration
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WB
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Mouse
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Citation
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Intracellular angiopoietin-1 promotes TKI-resistance via activation of JAK/STAT5 pathway in chronic myeloid leukemia
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WB
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Human
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Citation
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