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DGCR8 Recombinant Rabbit Monoclonal Antibody [ST04-79]

Usd: 385

Specification

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Catalog# ET1609-32

DGCR8 Recombinant Rabbit Monoclonal Antibody [ST04-79]

Application

  • WB

  • IF-Cell

  • IF-Tissue

  • IP

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

DGCR8 Recombinant Rabbit Monoclonal Antibody [ST04-79]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human DGCR8 aa 383-773 / 773.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell, IF-Tissue, IP

Molecular Weight

Predicted band size: 86 kDa

Positive Control

PC12 cell lysate, NIH/3T3 cell lysate, Hela cell lysate, Hela, PC12.

Conjugation

unconjugated

Clone Number

ST04-79

RRID

AB_3069843

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:2,000

  • IF-Cell

  • 1:50-1:200

  • IF-Tissue

  • 1:50-1:200

  • IP

  • Use at an assay dependent concentration.

Target

Function

Component of the microprocessor complex that acts as a RNA- and heme-binding protein that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs.The heme-bound DGCR8 dimer binds pri-miRNAs as a cooperative trimer (of dimers) and is active in triggering pri-miRNA cleavage, whereas the heme-free DGCR8 monomer binds pri-miRNAs as a dimer and is much less active. Both double-stranded and single-stranded regions of a pri-miRNA are required for its binding.Specifically recognizes and binds N6-methyladenosine (m6A)-containing pri-miRNAs, a modification required for pri-miRNAs processing.Involved in the silencing of embryonic stem cell self-renewal.

Background References

1. Ho, JJ. et al. 2012. Functional importance of dicer protein in the adaptive cellular response to hypoxia. J. Biol. Chem. 287: 29003-29020.

2. Bellemer, C. et al. 2012. Microprocessor dynamics and interactions at endogenous imprinted C19MC microRNA genes. J. Cell. Sci. 125: 2709-2720.

Tissue Specificity

Ubiquitously expressed.

Subcellular Location

Nucleus, cytoplasm.

UNIPROT

SwissProt: Q8WYQ5 Human

SwissProt: Q9EQM6 Mouse

Unigene: 17134 Rat

Synonyms

DGCRK6 antibody

C22orf12 antibody

D16H22S788E antibody

D16Wis2 antibody

DGCR 8 antibody

Dgcr8 antibody

DGCR8 microprocessor complex subunit antibody

DGCR8_HUMAN antibody

DGCRK 6 antibody

DiGeorge syndrome critical region 8 antibody

Expand

Images

  • Western blot analysis of DGCR8 on different lysates using anti-DGCR8 antibody at 1/1,000 dilution.<br /><span style="font-weight: bold;">Positive control:</span> <br />Lane 1: Hela cell lysate <br />Lane 2: PC12 cell lysate<br />Lane 3: NIH/3T3 cell lysate

    Western blot analysis of DGCR8 on different lysates using anti-DGCR8 antibody at 1/1,000 dilution.
    Positive control:
    Lane 1: Hela cell lysate
    Lane 2: PC12 cell lysate
    Lane 3: NIH/3T3 cell lysate

  • ICC staining DGCR8 (1/200) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining DGCR8 (1/200) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining DGCR8 (1/200) in PC12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining DGCR8 (1/200) in PC12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation