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TNF alpha

Product code: R1203-1


  • Overview

  • Product name TNF alpha
  • Molecular Wt 17 kDa
  • Description TNF-α, the prototypical member of the TNF protein superfamily, is a homotrimeric type-II membrane protein. Membrane bound TNF-α is cleaved by the metalloprotease TACE/ADAM17 to generate a soluble homotrimer. Both membrane and soluble forms of TNF-α are biologically active. TNF-α is produced by a variety of immune cells including T cells, B cells, NK cells and macrophages. TNF-α plays a key regulatory role in infammation and host defense against bacterial infection, notably Mycobacterium tuberculosis. The antibody detects active recombinant murine TNF alpha. The detective sensitivity by Western Blot is 5ng.
  • Species reactivity Mouse
  • Tested applications WB,ELISA
  • Antibody type Rabbit Polyclonal Antibody
  • Immunogen peptide
  • Positive control Recombinant murine TNF alpha
  • Conjugate Non-conjugated
  • Alternative names APC1 antibody
    APC1 protein antibody
    Cachectin antibody
    DIF antibody
    Differentiation inducing factor antibody
    Macrophage cytotoxic factor antibody
    Tnf antibody
    TNF superfamily, member 2 antibody
    TNF, macrophage derived antibody
    TNF, monocyte derived antibody
    TNF-a antibody
    TNF-alpha antibody
    TNFA antibody
    TNFA_HUMAN antibody
    TNFSF2 antibody
    Tumor necrosis factor alpha antibody
    Tumor necrosis factor antibody
    Tumor necrosis factor ligand superfamily member 2 antibody
    Tumor Necrosis Factor, Membrane Form antibody
    Tumor necrosis factor, soluble form antibody
  • Properties

  • Form Liquid
  • Concentration 1 mg/mL.
  • Storage instructions Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
  • Buffer 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
  • Isotype IgG
  • Purity Immunogen affinity purified
  • Subcellular location Secreted, membrane
  • Database links P06804
  • Applications

    WB: 1:500-1:1,000
    ELISA: 1:10,000-20,000

  • Publishing research using TNF alpha? Please let us know so that we can cite the reference in this datasheet.

  • Sun, Q., Zhu, J., Cao, F., & Chen, F. (2017). Anti-inflammatory properties of extracts from Chimonanthus nitens Oliv. leaf. PLoS One, 12(7), e0181094. PMID: 28700722

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Specific Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

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