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CRISPR-Cas9 [8-A6-6]

Product code: EM50804


  • Overview

  • Product name CRISPR-Cas9
  • Description The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) Type II system is currently the most commonly used RNA-Guided Endonuclease technology for genome engineering. There are two distinct components to this system: (1) a guide RNA and (2) an endonuclease, in this case the CRISPR associated (Cas) nuclease, Cas9. The gRNA/Cas9 complex is recruited to the target sequence by the base-pairing between the gRNA sequence and the complement to the target sequence in the genomic DNA. For successful binding of Cas9, the genomic target sequence must also contain the correct Protospacer Adjacent Motiff (PAM) sequence immediately following the target sequence.
  • Tested applications WB,ICC
  • Antibody type Mouse Monoclonal Antibody
  • Immunogen recombinant protein
  • Positive control Human 293T cells transfected with CRISPR-Cas9 lysate.
  • Conjugate Non-conjugated
  • Clone number 8-A6-6
  • Alternative names Cas9 antibody
    CRISPR-associated endonuclease Cas9/Csn1 antibody
    CRISPR-Cas9/Csn1 antibody
    csn1 antibody
    SpyCas9 antibody
  • Properties

  • Form Liquid
  • Concentration 2 mg/mL.
  • Storage instructions Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
  • Buffer 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
  • Isotype IgM
  • Purity ProA affinity purified
  • Applications

    WB: 1:5,000
    ICC: 1:200

  • Publishing research using CRISPR-Cas9? Please let us know so that we can cite the reference in this datasheet.

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Specific Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols
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