Tubulin is one of several members of a small family of globular proteins. The most common members of the tubulin family are α-tubulin and β-tubulin. The beta-tubulin (relative molecular weight about 50 kDa) is counterpart of alpha-tubulin in tubulin heterodimer, it is coded by multiple tubulin genes and it is also posttranslationally modified. Heterogeneity of subunit is concentrated in C-terminal structural domain. Beta-Tubulin may have bound GTP or GDP. Under certain conditions β-tubulin can hydrolyze its bound GTP to GDP plus Pi, release the Pi, and exchange the GDP for GTP.
Mouse Monoclonal Antibody
NCCIT, NIH/3T3, PC12, Mouse heart, F9, zebrafish brain, Hela HepG2, Mouse brain
Beta 4 tubulin antibody
Beta 5 tubulin antibody
tubulin beta 2A antibody
Tubulin beta chain antibody
Tubulin beta-5 chain antibody
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
ProA affinity purified
Fig1: Western blot analysis of β-tubulin on different cell lysates using anti-β-tubulin antibody at 1/5000 dilution. Positive control:
Lane 1: NCCIT
Lane 2: NIH/3T3
Lane 3: PC12
Lane 4: Mouse heart
Lane 5: F9
Lane 6: zebrafish brain
Lane 7: Hela
Fig2: ICC staining β-tubulin in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC stainingβ-tubulin in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC stainingβ-tubulin in NIH/3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-β-tubulin antibody. Counter stained with hematoxylin.
Fig6: Flow cytometric analysis of HeLa?cells with β-tubulin antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgG (FITC) was used as the secondary antibody.
Fig7: Western blot analysis of β-tubulin on hybrid fish (crucian-carp) brain tissue lysate using anti-β-tubulin antibody at 1/500 dilution.
Publishing research using β-tubulin? Please let us know so that we can cite the reference in this datasheet.
Wang, Q., Chen, Q., Zhu, L., Chen, M., Xu, W., Panday, S., . . . Zhou, J. (2017). JWA regulates TRAIL-induced apoptosis via MARCH8-mediated DR4 ubiquitination in cisplatin-resistant gastric cancer cells. Oncogenesis, 6(7), e353.
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To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
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