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MiTF [JF100-01]

Product code: ET1702-86


  • Overview

  • Product name MiTF
  • Molecular Wt 55 kDa
  • Description MITF (microphthalmia-associated transcription factor) is a melanocytic nuclear protein that contains basic helix-loop-helix (HLH) and leucine zipper (LZ) domains. These protein motifs are frequently observed in other transcription factors and are particularly common to members of the Myc family. MITF can directly associate with DNA as a homodimer and is required for the development and differentiation of melanocytes. Its expression is upregulated by cAMP and cAMP-dependent pathways. MITF activates several different gene promoters by binding to their E-boxes. Tyrosinase, TRP1 and TRP2 are pigment synthesis genes activated by MITF. When MITF is phosphorylated on Ser73 (via the MAPK pathway), it associates with co-activators of the p300/CBP family and enhances transcription. MITF has several isoforms including MITF-M which is specifically expressed in melanocytes. In MITF-deficient mice there is a complete absence of melanocytes.
  • Species reactivity Human, Mouse, Rat
  • Tested applications WB,ICC,FC
  • Antibody type Recombinant Rabbit Monoclonal Antibody
  • Immunogen recombinant protein
  • Positive control A431, NIH/3T3, Hela, PC-12.
  • Conjugate Non-conjugated
  • Clone number JF100-01
  • Alternative names BHLHE32 antibody
    bHLHe32 antibody
    Class E basic helix-loop-helix protein 32 antibody
    CMM8 antibody
    Homolog of mouse microphthalmia antibody
    Mi antibody
    Microphthalmia associated transcription factor antibody
    Microphthalmia, mouse, homolog of antibody
    Microphthalmia-associated transcription factor antibody
    MITF antibody
    MITF_HUMAN antibody
    mitfa antibody
    nacre antibody
    WS2 antibody
    WS2A antibody
    z3A.1 antibody
  • Properties

  • Form Liquid
  • Concentration 1 mg/mL.
  • Storage instructions Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
  • Buffer 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
  • Isotype IgG
  • Purity ProA affinity purified
  • Subcellular location Nucleus.
  • Database links O75030(Human)
    Q08874(Mouse)
    O88368(Rat)
  • Applications

    WB: 1:1,000-1:2,000
    FC: 1:50-1:100
    ICC: 1:100-1:500

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Specific Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

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