MAP (mitogen-activated protein) kinases play a significant role in many biological processes, including cell adhesion and spreading, cell differentiation and apoptosis. p38α, p38β and p38γ, also known as MAPK14, MAPK11 and MAPK12, respectively, each contain one protein kinase domain and belong to the MAP kinase family. Expressed in different areas throughout the body with common expression patterns in heart, p38 proteins use magnesium as a cofactor to catalyze the ATP-dependent phosphorylation of target proteins. Via their catalytic activity, p38α, p38β and p38γ are involved in a variety of events throughout the cell, including signal transduction pathways, cytokine production and cell proliferation and differentiation. The p38 proteins are subject to phosphoryation on Thr and Tyr residues, an event which is thought to activate the phosphorylated protein.
Human, Mouse, Rat
Recombinant Rabbit Monoclonal Antibody
NIH/3T3, 293T, Hela, 293, human brain tissue, mouse heart tissue.
CSAID-binding protein antibody
Csaids binding protein antibody
Cytokine suppressive anti-inflammatory drug-binding protein antibody
MAP kinase 14 antibody
MAP kinase MXI2 antibody
MAP kinase p38 alpha antibody
MAPK 14 antibody
MAX-interacting protein 2 antibody
Mitogen-activated protein kinase 14 antibody
Mitogen-activated protein kinase p38 alpha antibody
p38 MAP kinase antibody
p38 mitogen activated protein kinase antibody
p38alpha Exip antibody
Stress-activated protein kinase 2a antibody
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Protein A purified.
Fig1: Western blot analysis of p38 MAPK on different lysates using anti-p38 MAPK antibody at 1/1,000 dilution.
Lane 1: NIH/3T3
Lane 2: Human brain
Lane 3: 293
Fig2: ICC staining p38 MAPK in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining p38 MAPK in NIH/3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining p38 MAPK in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-p38 MAPK antibody. Counter stained with hematoxylin.
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To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
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