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Histone H3 [JJ092-08]

Product code: ET1701-81

  • Overview

  • Product name Histone H3
  • Molecular Wt 15 kDa
  • Description In eukaryotes, DNA is wrapped around histone octamers to form the basic unit of chromatin structure. The octamer is composed of histones H2A, H2B, H3 and H4, and it associates with approximately 200 base pairs of DNA to form the nucleosome. The association of DNA with histones results in dense packing of chromatin, which restricts proteins involved in gene transcription from binding to DNA. p300 preferentially acetylates Histone H3 at lysines 14 and 18 and Histone H4 at lysines 5 and 8. PCAF in its native form, primarily acetylates Histone H3 at lysine 14 to a monoacetylated form, and less efficiently acetylates Histone H4 at lysine 8. Histone H4 may also be acetylated at lysines 12 and 16, and the involvement of acetylated H4 with Histones H2A, H2B and H3 suggests that acetylated histones may be involved in dynamic chromatin remodeling.
  • Species reactivity Human, Mouse, Rat
  • Tested applications WB,ICC/IF,IHC,FC
  • Antibody type Recombinant Rabbit Monoclonal Antibody
  • Immunogen recombinant protein
  • Positive control NIH/3T3, MCF-7, Hela, RH-35, human tonsil tissue, human liver tissue, human kidney tissue, human uterus tissue, mouse liver tissue.
  • Conjugate Non-conjugated
  • Clone number JJ092-08
  • Alternative names H3 histone family, member A antibody
    H3/A antibody
    H31_HUMAN antibody
    H3FA antibody
    Hist1h3a antibody
    HIST1H3B antibody
    HIST1H3C antibody
    HIST1H3D antibody
    HIST1H3E antibody
    HIST1H3F antibody
    HIST1H3G antibody
    HIST1H3H antibody
    HIST1H3I antibody
    HIST1H3J antibody
    histone 1, H3a antibody
    Histone cluster 1, H3a antibody
    Histone H3.1 antibody
    Histone H3/a antibody
    Histone H3/b antibody
    Histone H3/c antibody
    Histone H3/d antibody
    Histone H3/f antibody
    Histone H3/h antibody
    Histone H3/i antibody
    Histone H3/j antibody
    Histone H3/k antibody
    Histone H3/l antibody
  • Properties

  • Form Liquid
  • Concentration 1 mg/mL.
  • Storage instructions Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
  • Buffer 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
  • Isotype IgG
  • Purity ProA affinity purified
  • Subcellular location Nucleus, Chromosome.
  • Database links P68431(Human)
  • Applications

    WB: 1:1,000-1:2,000
    ICC: 1:50-1:200
    IHC: 1:50-1:200
    FC: 1:50-1:100

  • Publishing research using Histone H3? Please let us know so that we can cite the reference in this datasheet.

  • Wu, W., Wang, S., Liu, Q., Wang, X., Shan, T., & Wang, Y. (2018). Cathelicidin-WA attenuates LPS-induced inflammation and redox imbalance through activation of AMPK signaling. Free Radic Biol Med, 129, 338-353. PMID: 30273672
  • Qing, Z., Xiao-Hui, W., Xi-Mei, W., & Chao-Chun, Z. (2018). Vitamin C deficiency aggravates tumor necrosis factor alpha-induced insulin resistance. Eur J Pharmacol, 829, 1-11 PMID: 29625084
  • Shen, J.et al. (2018). Regulation of Vascular Calcification by Growth Hormone-Releasing Hormone and Its Agonists. Circ Res, 122(10), 1395-1408. doi: 10.1161/CIRCRESAHA.117.312418 PMID: 29618597
  • Shu, X., Wang, M., Xu, H., Liu, Y., Huang, J., Yao, Z., & Zhang, L. (2017). Extracts of Salvia-Nelumbinis Naturalis Ameliorate Nonalcoholic Steatohepatitis via Inhibiting Gut-Derived Endotoxin Mediated TLR4/NF-kappaB Activation. Evid Based Complement Alternat Med, 2017, 9208314. PMID: 28831287
  • Wang, K., Jin, X., Chen, Y., Song, Z., Jiang, X., Hu, F., . . . Topping, D. L. (2016). Polyphenol-Rich Propolis Extracts Strengthen Intestinal Barrier Function by Activating AMPK and ERK Signaling. Nutrients, 8(5). PMID: 27164138
  • Wang, J., Zhang, Y., Weng, W., Qiao, Y., Ma, L., Xiao, W., . . . Sun, F. (2013). Impaired phosphorylation and ubiquitination by p70 S6 kinase (p70S6K) and Smad ubiquitination regulatory factor 1 (Smurf1) promote tribbles homolog 2 (TRIB2) stability and carcinogenic property in liver cancer. J Biol Chem, 288(47), 33667-33681. doi: 10.1074/jbc.M113.503292 PMID: 24089522

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Specific Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

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