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Histone H2A(hydroxyl Y39) [SR4-17]

Product code: ET1602-35


  • Overview

  • Product name Histone H2A(hydroxyl Y39)
  • Molecular Wt 14 kDa
  • Description Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene, that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.
  • Species reactivity Human, Mouse, Rat
  • Tested applications WB,IHC
  • Antibody type Recombinant Rabbit Monoclonal Antibody
  • Immunogen recombinant protein
  • Positive control NIH/3T3, Hela, human tonsil tissue, human colon cancer tissue, mouse testis tissue, mouse colon tissue.
  • Conjugate Non-conjugated
  • Clone number SR4-17
  • Alternative names H2A/m antibody
    H2A1B_HUMAN antibody
    HIST1H2AE antibody
    Histone H2A type 1-B/E antibody
    Histone H2A.2 antibody
    Histone H2A.m antibody
    Histone H2A/a antibody
    Histone H2A/m antibody
  • Properties

  • Form Liquid
  • Concentration 1 mg/mL.
  • Storage instructions Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
  • Buffer 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
  • Isotype IgG
  • Purity ProA affinity purified
  • Subcellular location NIH/3T3, Hela, human tonsil tissue, human colon cancer tissue, mouse testis tissue, mouse colon tissue.
  • Database links P04908(Human)
    P28001(Human)
    P35065(Human)
    Q93077(Human)
    Q99878(Human)
    P10812(Mouse)
    P22752(Mouse)
    P02262(Rat)
  • Applications

    WB: 1:1,000-1:2,000
    IHC: 1:50-1:200

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Specific Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

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