The 14-3-3 family of proteins are important regulatory molecules ubiquitiously expressed in all eukarryotic cells which bind to numerous signaling proteins in various pathways driving critical cellular pathways of apoptosis, differentiation and cell cycle. 14-3-3 theta specifically has also been shown to play an important regulatory role in the TLR2 signaling pathways as a negative regulator of TLR2 ligand Pam3CySk4 induced NF-kB activation. 14-3-3 theta has previously been shown to interact with TLR4 ligand and MyD88 dependent phosphorylated PkC epsilon. 14-3-3 theta in the TLR4 signaling pathway is a positive regulator controlling release of IRF3 indcuded pro-inflammatory cytokines RANTES and IP-10. Currently identified by mass spec as part of the TLR2 signaling complex and taken along with TLR4 data, a 14-3-3 theta antibody can be used to examine the different regulatory functions of 14-3-3 theta for different TLRs through its interaction with common or unique TLR signaling adaptor molecules in addition to MyD88 or PkC epsilon such as TRAM or TRIF allowing further clarification of TLR specific pathway regulation.
Human, Mouse, Rat
Recombinant Rabbit Monoclonal Antibody
NIH/3T3, HT29, human skin tissue, mouse skin tissue.
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
ProA affinity purified
Fig1: Western blot analysis of 14-3-3 on different lysates using anti-14-3-3 antibody at 1/1,000 dilution. Positive control:
Lane 1: HT29
Lane 2: Human skin
Lane 3: NIH/3T3
Fig2: Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-14-3-3 antibody. Counter stained with hematoxylin.
Fig3: Flow cytometric analysis of SH-SY-5Y cells with 14-3-3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
1. Lehmann LH et al. Essential role of sympathetic endothelin A receptors for adverse cardiac remodeling. Proc Natl Acad Sci U S A 111:13499-504 (2014).
2. Dettmer U et al. In vivo cross-linking reveals principally oligomeric forms of a-synuclein and ?-synuclein in neurons and non-neural cells. J Biol Chem 288:6371-85 (2013).
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To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
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