Location: Home >Product >Product Detail

Phospho-Raf1 (S259) [SD85-07]

Product code: ET1612-87


  • Overview

  • Product name Phospho-Raf1 (S259)
  • Molecular Wt 73 kDa
  • Description Raf-1 is a ubiquitously expressed cytoplasmic protein with intrinsic serine/threonine kinase activity. Raf-1, or c-Raf, is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein is associated with cellular transformation and mitogenesis. Raf-1 is normally suppressed by its regulatory N-terminal domain. Raf-1 is activated in response to a variety of tyrosine kinase receptors as well as in response to pp60v-Src expression. Specifically, Raf-1 is phosphorylated in the catalytic domain at Ser 338 and, to a lesser extent, Ser 339. This phosphorylation requires the co-activation of PI 3-kinase and the Ras signaling pathway. Raf-1 is also phosphorylated on Tyr 340 and 341, which induces the phosphorylation of MEK. Phosphorylation of Ser 621 is essential for the catalytic activity of Raf-1 and downregulation by c-AMP-dependent protein kinase A (PKA). PKA also phosphorylates Raf-1 on Ser 43 and Ser 259. PKA phosphorylation of Ser 259 inhibits Raf-1 and decreases the phosphorylation necessary for Raf-1 activation at Ser 338.
  • Species reactivity Human, Mouse, Rat
  • Tested applications WB,IHC
  • Antibody type Recombinant Rabbit Monoclonal Antibody
  • Immunogen Synthetic phospho-peptide corresponding to residues surrounding Ser259 of human Raf1
  • Positive control Hela, human skeletal muscle tissue.
  • Conjugate Non-conjugated
  • Clone number SD85-07
  • Alternative names c Raf antibody
    C-raf antibody
    C-Raf proto-oncogene, serine/threonine kinase antibody
    CMD1NN antibody
    Craf 1 transforming gene antibody
    cRaf antibody
    Craf1 transforming gene antibody
    EC 2.7.11.1 antibody
    kinase Raf1 antibody
    Murine sarcoma 3611 oncogene 1 antibody
    NS5 antibody
    Oncogene MIL antibody
    Oncogene RAF1 antibody
    OTTHUMP00000160218 antibody
    OTTHUMP00000207813 antibody
    OTTHUMP00000209389 antibody
    Protein kinase raf 1 antibody
    Proto-oncogene c-RAF antibody
    Raf 1 antibody
    Raf 1 proto oncogene serine/threonine kinase antibody
    RAF antibody
    Raf proto oncogene serine/threonine protein kinase antibody
    RAF proto-oncogene serine/threonine-protein kinase antibody
    RAF-1 antibody
    RAF1 antibody
    RAF1_HUMAN antibody
    Similar to murine leukemia viral (V-raf-1) oncogene homolog 1 antibody
    TRANSFORMING REPLICATION-DEFECTIVE MURINE RETROVIRUS 3611-MSV antibody
    v raf 1 murine leukemia viral oncogene homolog 1 antibody
    v-raf murine sarcoma viral oncogene homolog 1 antibody
    v-raf-1 murine leukemia viral oncogene-like protein 1 antibody
    vraf1 murine leukemia viral oncogene homolog 1 antibody
  • Properties

  • Form Liquid
  • Concentration 1 mg/mL.
  • Storage instructions Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
  • Buffer 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
  • Isotype IgG
  • Purity ProA affinity purified
  • Subcellular location Cytoplasm, Cell membrane, Mitochondrion, Nucleus.
  • Database links P04049(Human)
    Q99N57(Mouse)
    P11345(Rat)
  • Applications

    WB: 1:1,000-1:2,000
    IHC: 1:50-1:200

  • Publishing research using Phospho-Raf1 (S259)? Please let us know so that we can cite the reference in this datasheet.

  • Data sorting...

Choose your product,application and species below,then click "Create".

On the next page you can complete and submit your review.

  • * Code NO.
  • * Application
  • *Sample
  • Species Tested
  • Against
  • Choose text
  • Content

Leave us a review

Specific Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols
Add to cart successfully.