Raf-1 is a ubiquitously expressed cytoplasmic protein with intrinsic serine/threonine kinase activity. Raf-1, or c-Raf, is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein is associated with cellular transformation and mitogenesis. Raf-1 is normally suppressed by its regulatory N-terminal domain. Raf-1 is activated in response to a variety of tyrosine kinase receptors as well as in response to pp60v-Src expression. Specifically, Raf-1 is phosphorylated in the catalytic domain at Ser 338 and, to a lesser extent, Ser 339. This phosphorylation requires the co-activation of PI 3-kinase and the Ras signaling pathway. Raf-1 is also phosphorylated on Tyr 340 and 341, which induces the phosphorylation of MEK. Phosphorylation of Ser 621 is essential for the catalytic activity of Raf-1 and downregulation by c-AMP-dependent protein kinase A (PKA). PKA also phosphorylates Raf-1 on Ser 43 and Ser 259. PKA phosphorylation of Ser 259 inhibits Raf-1 and decreases the phosphorylation necessary for Raf-1 activation at Ser 338.
Human, Mouse, Rat
Recombinant Rabbit Monoclonal Antibody
Synthetic phospho-peptide corresponding to residues surrounding Ser259 of human Raf1
Hela, human skeletal muscle tissue.
c Raf antibody
C-Raf proto-oncogene, serine/threonine kinase antibody
Craf 1 transforming gene antibody
Craf1 transforming gene antibody
EC 22.214.171.124 antibody
kinase Raf1 antibody
Murine sarcoma 3611 oncogene 1 antibody
Oncogene MIL antibody
Oncogene RAF1 antibody
Protein kinase raf 1 antibody
Proto-oncogene c-RAF antibody
Raf 1 antibody
Raf 1 proto oncogene serine/threonine kinase antibody
Raf proto oncogene serine/threonine protein kinase antibody
RAF proto-oncogene serine/threonine-protein kinase antibody
Similar to murine leukemia viral (V-raf-1) oncogene homolog 1 antibody
TRANSFORMING REPLICATION-DEFECTIVE MURINE RETROVIRUS 3611-MSV antibody
v raf 1 murine leukemia viral oncogene homolog 1 antibody
v-raf murine sarcoma viral oncogene homolog 1 antibody
v-raf-1 murine leukemia viral oncogene-like protein 1 antibody
vraf1 murine leukemia viral oncogene homolog 1 antibody
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
ProA affinity purified
Cytoplasm, Cell membrane, Mitochondrion, Nucleus.
Fig1: Western blot analysis of Phospho-Raf1(S259) on different lysates using anti-Phospho-Raf1(S259) antibody at 1/1,000 dilution. Positive control:
Lane 1: Human skeletal muscle
Lane 2: Hela
Fig2: Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-Phospho-Raf1(S259) antibody. Counter stained with hematoxylin.
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