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Phospho-Raf1 (S259) [SD85-07]

Product code: ET1612-87

  • Overview

  • Product name Phospho-Raf1 (S259)
  • Molecular Wt 73 kDa
  • Description Raf-1 is a ubiquitously expressed cytoplasmic protein with intrinsic serine/threonine kinase activity. Raf-1, or c-Raf, is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein is associated with cellular transformation and mitogenesis. Raf-1 is normally suppressed by its regulatory N-terminal domain. Raf-1 is activated in response to a variety of tyrosine kinase receptors as well as in response to pp60v-Src expression. Specifically, Raf-1 is phosphorylated in the catalytic domain at Ser 338 and, to a lesser extent, Ser 339. This phosphorylation requires the co-activation of PI 3-kinase and the Ras signaling pathway. Raf-1 is also phosphorylated on Tyr 340 and 341, which induces the phosphorylation of MEK. Phosphorylation of Ser 621 is essential for the catalytic activity of Raf-1 and downregulation by c-AMP-dependent protein kinase A (PKA). PKA also phosphorylates Raf-1 on Ser 43 and Ser 259. PKA phosphorylation of Ser 259 inhibits Raf-1 and decreases the phosphorylation necessary for Raf-1 activation at Ser 338.
  • Species reactivity Human, Mouse, Rat
  • Tested applications WB,IHC
  • Antibody type Recombinant Rabbit Monoclonal Antibody
  • Immunogen Synthetic phospho-peptide corresponding to residues surrounding Ser259 of human Raf1
  • Positive control Hela, human skeletal muscle tissue.
  • Conjugate Non-conjugated
  • Clone number SD85-07
  • Alternative names c Raf antibody
    C-raf antibody
    C-Raf proto-oncogene, serine/threonine kinase antibody
    CMD1NN antibody
    Craf 1 transforming gene antibody
    cRaf antibody
    Craf1 transforming gene antibody
    EC antibody
    kinase Raf1 antibody
    Murine sarcoma 3611 oncogene 1 antibody
    NS5 antibody
    Oncogene MIL antibody
    Oncogene RAF1 antibody
    OTTHUMP00000160218 antibody
    OTTHUMP00000207813 antibody
    OTTHUMP00000209389 antibody
    Protein kinase raf 1 antibody
    Proto-oncogene c-RAF antibody
    Raf 1 antibody
    Raf 1 proto oncogene serine/threonine kinase antibody
    RAF antibody
    Raf proto oncogene serine/threonine protein kinase antibody
    RAF proto-oncogene serine/threonine-protein kinase antibody
    RAF-1 antibody
    RAF1 antibody
    RAF1_HUMAN antibody
    Similar to murine leukemia viral (V-raf-1) oncogene homolog 1 antibody
    v raf 1 murine leukemia viral oncogene homolog 1 antibody
    v-raf murine sarcoma viral oncogene homolog 1 antibody
    v-raf-1 murine leukemia viral oncogene-like protein 1 antibody
    vraf1 murine leukemia viral oncogene homolog 1 antibody
  • Properties

  • Form Liquid
  • Concentration 1 mg/mL.
  • Storage instructions Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
  • Buffer 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
  • Isotype IgG
  • Purity ProA affinity purified
  • Subcellular location Cytoplasm, Cell membrane, Mitochondrion, Nucleus.
  • Database links P04049(Human)
  • Applications

    WB: 1:1,000-1:2,000
    IHC: 1:50-1:200

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Specific Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

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