The Drosophila discs large (dlg) tumor suppressor gene was first identified in Drosophila through genetic analysis of germline mutations. Several mammalian homologs were subsequently identified and categorized into a protein family termed MAGUK (membrane-associated guanylate kinase homolog). Human homologs of dlg include hdlg-1 (rat SAP 97) and NE-dlg (neuronal and endocrine dlg). The rat synaptic protein PSD-95 (also designated SAP 90) also shares homology with these proteins. MAGUKs are localized at the membrane-cytoskeleton interface and contain several distinct domains which suggest a role for these proteins in intracellular signal transduction. Interaction of hdlg-1 and NE-dlg with the tumor suppresor protein APC suggest that MAGUK proteins may also play a role in regulation of growth.
Human, Mouse, Rat
Recombinant Rabbit Monoclonal Antibody
N2A, SH-SY-5Y, rat brain tissue, mouse cerebullum tissue, rat cerebellum tissue.
Discs large homolog 4 antibody
Disks large homolog 4 antibody
DLG 4 antibody
Human post synaptic density protein 95 antibody
Post synaptic density protein 95 antibody
Postsynaptic density protein 95 antibody
PSD 95 antibody
SAP 90 antibody
Synapse associated protein 90 antibody
Synapse-associated protein 90 antibody
Tax interaction protein 15 antibody
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
ProA affinity purified
Cell membrane, Cell junction
Fig1: Western blot analysis of PSD95 on different lysates using anti-PSD95 antibody at 1/1,000 dilution. Positive control:
Lane 1: Mouse brain
Lane 2: Human brain
Fig2: ICC staining PSD95 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining PSD95 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-PSD95 antibody. Counter stained with hematoxylin.
Fig5: Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-PSD95 antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded mouse cerebullum tissue using anti-PSD95 antibody. Counter stained with hematoxylin.
Fig7: Flow cytometric analysis of SH-SY-5Y cells with PSD95 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
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