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SUPT5H [JE55-25]

Product code: ET7111-01


  • Overview

  • Product name SUPT5H
  • Molecular Wt Predicted band size: 121 kDa.
  • Description Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences.
  • Species reactivity Human,Mouse,Rat
  • Tested applications WB,ICC,IHC-P
  • Antibody type Recombinant Rabbit Monoclonal Antibody
  • Immunogen Synthetic peptide within C-terminal Human SPT5.
  • Positive control Human placenta tissue lysate, rat skin tissue lysate, F9 cell lysate, A549, MCF-7, rat large intestine tissue, human cervix tissue, human thyroid tissue, human colon carcinoma tissue, human skin tissue, human prostate carcinoma tissue, human breast carcinoma tissue, human stomach carcinoma tissue, human small intestine tissue, human pancreas tissue, mouse colon tissue, mouse heart tissue.
  • Conjugate Non-conjugated
  • Clone number JE55-25
  • Alternative names DRB sensitivity inducing factor large subunit antibody
    DRB sensitivity-inducing factor 160 kDa subunit antibody
    DRB sensitivity-inducing factor large subunit antibody
    DSIF large subunit antibody
    DSIF p160 antibody
    FLJ34157 antibody
    hSPT 5 antibody
    hSPT5 antibody
    SPT 5 antibody
    SPT 5H antibody
    SPT5 antibody
    SPT5H antibody
    SPT5H_HUMAN antibody
    Suppressor of Ty 5 homolog antibody
    Suppressor of Ty 5 homolog (S. cerevisiae) antibody
    SUPT 5H antibody
    supt5h antibody
    Tat cotransactivator 1 protein antibody
    Tat CT1 antibody
    Tat CT1 protein antibody
    Tat-cotransactivator 1 protein antibody
    Tat-CT1 protein antibody
    Transcription elongation factor SPT 5 antibody
    Transcription elongation factor spt5 antibody
    Transcription factor Tat CT1 antibody
  • Properties

  • Form Liquid
  • Concentration 1 mg/mL.
  • Storage instructions Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
  • Buffer 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
  • Isotype IgG
  • Purity Protein A affinity purified.
  • Subcellular location Nucleus.
  • Database links SwissProt: O00267 Human
    SwissProt: O55201 Mouse
  • Applications

    WB:1:500-1:2,000
    ICC:1:50-1:100
    IHC-P:1:50-1:200

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Specific Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

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