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HA tag Nanoselector Agarose

Product code: NBS09A


  • Overview

  • Product name HA tag Nanoselector Agarose
    • Introduction
    • An HA-tag is derived from the human influenza hemagglutinin (HA) molecule corresponding to amino acids 98-106. It has been extensively used as a general epitope tag in expression vectors. The HA-tag does not appear to interfere with the bioactivity or the biodistribution of the recombinant HA-tagged protein.The HA tag's amino acid sequence is: YPYDVPDYA
      HA tag Nanoselector Agarose is a ready to use resin for immunoprecipitation/pulldown of HA tag fusion proteins

    • Specificity
    • The HA -Nanoselector recognizes the HA-tag sequence motif YPYDVPDYA at the N-terminus, C-terminus, or internal site of the fusion protein.

    • Affinity
    • 1.3nM

    • Bead properties
    • Bead size: ~ 90 µm (cross-linked 4% agarose beads) Storage buffer: 20% EtOH

    • Binding capacity
    • High binding capacity,10 µL slurry bind about 20 µg of recombinant GFP-HA tag

    • Elution
    • - SDS sample buffer - 0.2 M glycine pH 2.5 -40 µM YPYDVPDYA peptide Instead of elution, we recommend on-bead assays like on-bead digestion for MS analysis.

    • Stability and Storage
    • Shipped at ambient temperature. Upon receipt store at +4°C. Stable for 1 year. Do not freeze

    • Benefits
    • Efficient immunoprecipitation of HA-tag fusion proteins, low background No heavy & light antibody chains in downstream applications Easy elution of native proteins Harsh washing conditions
  • Publishing research using HA tag Nanoselector Agarose? Please let us know so that we can cite the reference in this datasheet.

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Specific Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols
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