PDLIM1, also known as CLP36, CLIM1 or Elfin, is a 329 amino acid cytoplasmic protein that associates with Actin stress fibers at the cytoskeleton. Expressed at high levels in skeletal muscle and heart and at lower levels in colon, small intestine, spleen, lung, placenta, kidney, liver, thymus and pancreas, CLP36 functions as a cytoskeletal protein that is thought to act as an adaptor, bringing target proteins to the cytoskeleton. Specifically, CLP36 interacts with Clik1 (a kinase) and recruits Clik1 to α-actinin-1, thereby facilitating the association of Clik1 with Actin stress fibers. CLP36 contains one PDZ domain and one LIM zinc-binding domain through which it conveys its protein-protein binding capabilities. Human CLP36 shares 88% sequence similarity with its rat counterpart, suggesting a conserved function between species.
Recombinant Rabbit Monoclonal Antibody
Synthetic peptide within N-terminal Human PDLIM1.
Human placenta tissue lysate, mouse lung tissue lysate, HepG2 cell lysate, rat heart tissue, HepG2.
C terminal LIM domain protein 1 antibody
C-terminal LIM domain protein 1 antibody
Carboxy terminal LIM domain protein 1 antibody
CLIM 1 antibody
CLP 36 antibody
Enigma homolog antibody
hCLIM 1 antibody
LIM domain protein CLP 36 antibody
LIM domain protein CLP-36 antibody
LIM domain protein CLP36 antibody
PDLIM 1 antibody
PDZ and LIM domain 1 antibody
PDZ and LIM domain protein 1 antibody
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Protein A affinity purified.
Cytoskeleton, cytoplasm, Z line.
SwissProt: O00151 Human
SwissProt: O70400 Mouse
SwissProt: Q3TZ17 Mouse
SwissProt: P52944 Rat
Fig1: Western blot analysis of PDLIM1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7110-99, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Lane 1: Human placenta tissue lysate
Lane 2: Mouse lung tissue lysate
Lane 3: HepG2 cell lysate
Fig2: Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-PDLIM1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-99, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Fig3: Flow cytometric analysis of PDLIM1 was done on HepG2 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7110-99, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
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To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
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