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CD45 [JE03-05]

Product code: ET7111-03

  • Overview

  • Product name CD45
  • Molecular Wt Predicted band size: 147 kDa.
  • Description Protein tyrosine phosphatase, receptor type, C also known as PTPRC is an enzyme that, in humans, is encoded by the PTPRC gene. PTPRC is also known as CD45 antigen (CD stands for cluster of differentiation), which was originally called leukocyte common antigen (LCA). The protein product of this gene, best known as CD45, is a member of the protein tyrosine phosphatase (PTP) family. PTPs are signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. CD45 contains an extracellular domain, a single transmembrane segment, and two tandem intracytoplasmic catalytic domains, and thus belongs to the receptor type PTP family. CD45 is a type I transmembrane protein that is present in various isoforms on all differentiated hematopoietic cells (except erythrocytes and plasma cells). CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes via its extracellular domain (a form of co-stimulation), or by activating various Src family kinases required for the antigen receptor signaling via its cytoplasmic domain. CD45 also suppresses JAK kinases, and so functions as a negative regulator of cytokine receptor signaling.
  • Species reactivity Human
  • Tested applications WB,IHC,FC
  • Antibody type Recombinant Rabbit Monoclonal Antibody
  • Immunogen Synthetic peptide corresponding to the C-terminal human CD45.
  • Positive control Jurkat cell lysates, human tonsil tissue, human colon cancer tissue, human spleen cancer tissue, Jurkat.
  • Conjugate Non-conjugated
  • Clone number JE03-05
  • Alternative names B220 antibody
    CD 45 antibody
    CD45 antibody
    CD45 antigen antibody
    CD45R antibody
    GP180 antibody
    L-CA antibody
    LCA antibody
    Leukocyte common antigen antibody
    loc antibody
    Ly-5 antibody
    LY5 antibody
    Ly5, homolog of antibody
    Lyt-4 antibody
    OTTHUMP00000033813 antibody
    OTTHUMP00000033816 antibody
    OTTHUMP00000033817 antibody
    OTTHUMP00000038574 antibody
    Protein tyrosine phosphatase receptor type c polypeptide antibody
    Protein tyrosine phosphatase, receptor type C antibody
    protein tyrosine phosphatase, receptor type, C antibody
    Protein tyrosine phosphatase, receptor type, c polypeptide antibody
    Ptprc antibody
    PTPRC_HUMAN antibody
    Receptor-type tyrosine-protein phosphatase C antibody
    T200 antibody
    T200 glycoprotein antibody
    T200 leukocyte common antigen antibody
  • Properties

  • Form Liquid
  • Concentration 1 mg/ml.
  • Storage instructions Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
  • Buffer 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
  • Isotype IgG
  • Purity Protein A purified.
  • Subcellular location Cell junction, Cell membrane, Membrane
  • Database links SwissProt: P08575 Human
  • Applications

    WB: 1:500-1:1000
    IHC: 1:50-1:200
    FC: 1:50-1:100

  • Publishing research using CD45? Please let us know so that we can cite the reference in this datasheet.

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Specific Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

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