This gene is a member of the equilibrative nucleoside transporter family. The gene encodes a transmembrane glycoprotein that localizes to the plasma and mitochondrial membranes and mediates the cellular uptake of nucleosides from the surrounding medium. The protein is categorized as an equilibrative (as opposed to concentrative) transporter that is sensitive to inhibition by nitrobenzylthioinosine (NBMPR). Nucleoside transporters are required for nucleotide synthesis in cells that lack de novo nucleoside synthesis pathways, and are also necessary for the uptake of cytotoxic nucleosides used for cancer and viral chemotherapies. Multiple alternatively spliced variants, encoding the same protein, have been found for this gene.
Rabbit Polyclonal Antibody
Synthetic peptide within human ETN1 aa 1-80.
Human plasma lysates, F9, JAR.
Equilibrative NBMPR-sensitive nucleoside transporter antibody
equilibrative nitrobenzylmercaptopurine riboside (NBMPR)-sensitive nucleoside transporter antibody
Equilibrative nitrobenzylmercaptopurine riboside-sensitive nucleoside transporter antibody
Equilibrative nucleoside transporter 1 antibody
Nucleoside transporter antibody
Nucleoside transporter, es-type antibody
solute carrier family 29 (equilibrative nucleoside transporter), member 1 antibody
solute carrier family 29 (nucleoside transporters), member 1 antibody
Solute carrier family 29 member 1 antibody
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Peptide affinity purified.
SwissProt: Q99808 Human
SwissProt: Q9JIM1 Mouse
Fig1: Western blot analysis of ETN1 on Human plasma lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-23, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Fig2: ICC staining of ETN1 in F9 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1902-23, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Fig3: Flow cytometric analysis of ETN1 was done on JAR cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1902-23, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
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To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
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