This gene is a member of the leukocyte immunoglobulin-like receptor (LIR) family, which is found in a gene cluster at chromosomal region 19q13.4. The encoded protein belongs to the subfamily B class of LIR receptors which contain two or four extracellular immunoglobulin domains, a transmembrane domain, and two to four cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs). Several other LIR subfamily B receptors are expressed on immune cells where they bind to MHC class I molecules on antigen-presenting cells and inhibit stimulation of an immune response. Multiple transcript variants encoding different isoforms have been found for this gene.
Mouse Monoclonal Antibody
Purified recombinant fragment of human LILRB5 (AA: extra 24-164) expressed in E. Coli.
CD85 antigen-like family member C antibody
Leukocyte immunoglobulin-like receptor 8 antibody
Leukocyte immunoglobulin-like receptor subfamily B member 5 antibody
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
1*PBS with 0.05% sodium azide.
Protein G purified.
Fig1: Western blot analysis of LILRB5 against human LILRB5 (AA: extra 24-164) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-50, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Fig2: Western blot analysis of EM1711-50 against HEK293 (1) and LILRB5 (AA: extra 24-164)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-50, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Fig3: Flow cytometric analysis of LILRB5 was done on HL-60 cells . The cells were fixed, permeabilized and stained with the primary antibody (EM1711-50, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).
Publishing research using LILRB5? Please let us know so that we can cite the reference in this datasheet.
Choose your product,application and species below,then click "Create".
On the next page you can complete and submit your review.
Leave us a review
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Click here to view the general protocols