The innate immune system senses viral infection by recognizing many different viral components and triggering specific antiviral responses. Intracellular double-stranded RNA (dsRNA) is a major sign of replication for many viruses. Retinoic acid inducible gene I (RIG-I) is a 925 amino acid, interferon-inducible cellular DExD/H box RNA helicase that activates type I interferon (IFN), an important effector of the innate immune system that is sensitive to these dsRNA viruses. dsRNA is normally present in very low quantities in cells, so when a virus is present, the elevated levels of dsRNA act as a sign telling RIG-I to activate the production of IFN. RIG-I does this by using its helicase domain to bind to viral dsRNA, thus transmitting the activation signal for IFN through IkB kinase-related kinases and inducing IFN expression. RIG-I is expressed in the cytoplasm of fibroblasts and conventional dendritic cells and can distinguish between many different RNA viruses.
Mouse Monoclonal Antibody
Human DDX58 recombinant protein, DDX58-hIgGFc transfected HEK293 cell lysate, Hela, MCF-7.
DEAD (Asp Glu Ala Asp) box polypeptide 58 antibody
DEAD (Asp Glu Ala Asp/His) box polypeptide antibody
DEAD box protein 58 antibody
DEAD/H (Asp Glu Ala Asp/His) box polypeptide RIG1 antibody
Probable ATP dependent RNA helicase DDX58 antibody
Probable ATP-dependent RNA helicase DDX58 antibody
Retinoic acid inducible gene 1 protein antibody
Retinoic acid-inducible gene 1 protein antibody
Retinoic acid-inducible gene I protein antibody
RIG I antibody
RLR 1 antibody
RNA helicase antibody
RNA helicase RIG I antibody
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Protein A purified.
Fig1: Western blot analysis of DDX58 on human DDX58 recombinant protein using anti-DDX58 antibody at 1/1,000 dilution.
Fig2: Western blot analysis of DDX58 on HEK293 (1) and DDX58-hIgGFc transfected HEK293 (2) cell lysate using anti-DDX58 antibody at 1/1,000 dilution.
Fig3: ICC staining DDX58 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining DDX58 (green) and Actin filaments (red) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Flow cytometric analysis of Hela cells with DDX58 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
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To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
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