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ROBO1 [10E2]

Product code: EM1901-72


  • Overview

  • Product name ROBO1
  • Molecular Wt Predicted band size 170-181 kDa.
  • Description Specific cells in the midline separate the left and right halves of the central nervous system and play many roles in guiding the growth cone behavior. In vertebrate spinal cord, insect abdominal nerves and nematodes, midline cells produce induced cues, such as nectins and slits, respectively, as attractants and repellents. respectively. These cells can serve as gatekeepers to prevent axons from passing through the midline and to guide the growth cone in response to the switching of lead cues beyond the crossing. One such gatherer, Robo, is an axon guidance receptor that defines a new subfamily of proteins from fruit flies to mammalian conserved Ig superfamily proteins. Robo acts as a receptor for the repellent Slit and functions in a cell-autonomous manner Non-intersecting axons express high levels of Robo and cross axons to express low levels of Robo and then reach midline and high levels of crossover. Robo1 and Robo2 are two human homologs of Drosophila megalopolis. Robo1 is also homologous to the C. elegans gene sax3, while Robo2 is homologous to zebrafish genes.
  • Species reactivity Human,Mouse
  • Tested applications WB,IHC,ICC,FC
  • Antibody type Mouse Monoclonal Antibody
  • Immunogen Recombinant protein within human ROBO1 aa 40-300.
  • Positive control Siha cell lysates, SHSY5Y, Siha, human Liver cancer tissue, human kidney tissue, human stomach cancer tissue, mouse brain tissue.
  • Conjugate Non-conjugated
  • Clone number 10E2
  • Alternative names Deleted in U twenty twenty antibody
    DUTT 1 antibody
    DUTT1 antibody
    FLJ21882 antibody
    H Robo 1 antibody
    H-Robo-1 antibody
    hRobo 1 antibody
    Robo 1 antibody
    Robo1 antibody
    ROBO1_HUMAN antibody
    Roundabout 1 antibody
    Roundabout axon guidance receptor homolog 1 antibody
    Roundabout homolog 1 antibody
    Roundabout homolog1 precurser antibody
    Roundabout1 antibody
    SAX 3 antibody
    SAX3 antibody
  • Properties

  • Form Liquid
  • Concentration 2 mg/ml.
  • Storage instructions Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
  • Buffer 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
  • Isotype IgG1
  • Purity Protein G purified.
  • Subcellular location Membrane.
  • Database links SwissProt: Q9Y6N7 Human
  • Applications

    WB: 1:1000-1:5000
    IHC: 1:50-1:200
    ICC: 1:50-1:200
    FC: 1:50-1:100

  • Publishing research using ROBO1? Please let us know so that we can cite the reference in this datasheet.

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Specific Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

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