BASP1 is a brain abundant membrane bound protein with several transient phosphorylation sites and PEST motifs. BASP1 binds calmodulin, is phosphorylated by protein kinase C and undergoes N-terminal myristoylation. BASP1 is expressed specifically in nervous tissue. Intercellular signaling and nerve outgrowth are influenced by a number of proteins expressed at nerve endings and synapses of which BASP1 is one.
Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit
Rabbit Polyclonal Antibody
KLH conjugated synthetic peptide derived from human BASP1/Nap22 3-100/227
Brain abundant membrane attached signal protein 1
Brain Abundant Signal Protein Membrane Attached 1
Brain Acid Soluble Protein 1
Neuronal axonal membrane protein NAP 22
Neuronal tissue enriched acidic protein
22 kDa neuronal tissue enriched acidic protein
Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
affinity purified by Protein A
Cell membrane; Lipid-anchor. Cell projection, growth cone. Note=Associated with the membranes of growth cones that form the tips of elongating axons.
SwissProt: P80723 Human
Fig1: Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (BASP1) Polyclonal Antibody, Unconjugated (ER1904-13) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Fig2: Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-BASP1 Polyclonal Antibody, Unconjugated(ER1904-13) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Fig3: Sample:Hela (Human)Cell Lysate at 40 ug
Primary: Anti-BASP1(ER1904-13)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 25kD
Observed band size: 26kD
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To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
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