Involved primarily in ATP hydrolysis at the plasma membrane. Plays a role in regulating pyrophosphate levels, and functions in bone mineralization and soft tissue calcification. In vitro, has a broad specificity, hydrolyzing other nucleoside 5' triphosphates such as GTP, CTP, TTP and UTP to their corresponding monophosphates with release of pyrophosphate and diadenosine polyphosphates, and also 3',5'-cAMP to AMP. May also be involved in the regulation of the availability of nucleotide sugars in the endoplasmic reticulum and Golgi, and the regulation of purinergic signaling. Appears to modulate insulin sensitivity.
Human, Mouse, Rat, Dog, Pig, Horse, Rabbit
Rabbit Polyclonal Antibody
KLH conjugated synthetic peptide derived from human ENPP1 41-140/925
Expressed in plasma cells and also in a number of non-lymphoid tissues, including the distal convoluted tubule of the kidney, chondrocytes and epididymis.
Alkaline phosphodiesterase 1
Ectonucleotide pyrophosphatase/phosphodiesterase 1
Ectonucleotide pyrophosphatase/phosphodiesterase family member 1
Ly 41 antigen
Membrane component chromosome 6 surface marker 1
Phosphodiesterase I/nucleotide pyrophosphatase 1
Plasma cell membrane glycoprotein 1
Plasma-cell membrane glycoprotein PC-1.
Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
affinity purified by Protein A
Membrane; Single-pass type II membrane protein. Basolateral cell membrane; Single-pass type II membrane protein. Note=Targeted to the basolateral membrane in polarized epithelial cells and in hepatocytes, and to matrix vesicles in osteoblasts. In bile duct cells and cancer cells, located to the apical cytoplasmic side.
SwissProt: P22413 Human
Fig1: Sample: Testis (Mouse) Lysate at 40 ug
Kidney (Mouse) Lysate at 40 ug
Pancreas (Mouse) Lysate at 40 ug
Primary: Anti-ENPP1 (ER1908-13) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
Predicted band size: 100 kD
Observed band size: 100 kD
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