Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH1 complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Required for embryonic stem cell derivation and self-renewal, suggesting that it is involved in safeguarding embryonic stem cell identity. Compared to EZH1-containing complexes, it is less abundant in embryonic stem cells, has weak methyltransferase activity and plays a less critical role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation.
Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit, Guinea Pig
Rabbit Polyclonal Antibody
KLH conjugated synthetic peptide derived from human EZH1 471-510/747
293T(Human) Cell Lysate, Rat lung, Mouse brain.
Enhancer of zeste homolog 1(Drosophila)
Enhancer of zeste homolog 1
EZH1: enhancer of zeste homolog 1 (Drosophila)
Histone-lysine N-methyltransferase EZH1
Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
affinity purified by Protein A
Nucleus. Note=Colocalizes with trimethylated 'Lys-27' of histone H3.
SwissProt: Q92800 Human
293T(Human) Cell Lysate at 30 ug
Primary: Anti-EZH1 (ER1908-44) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 85 kD
Observed band size: 76 kD
Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (EZH1) Polyclonal Antibody, Unconjugated (ER1908-44) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Fig3: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (EZH1) Polyclonal Antibody, Unconjugated (ER1908-44) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Publishing research using EZH1? Please let us know so that we can cite the reference in this datasheet.
Choose your product,application and species below,then click "Create".
On the next page you can complete and submit your review.
Leave us a review
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Click here to view the general protocols