The karyopherin-related nuclear transport factor Exportin 5, also known as Exp5, preferentially recognizes and transports RNAs containing minihelix motifs, structural cis-acting export elements that comprise a double-stranded stem (14 nucleotides) with a base-paired 5' end and a 3-8-nucleotide protruding 3' end. Exportin 5 also mediates protein transport between the nuclear and cytoplasmic compartment. Exportin 5 belongs to a large family of karyopherins and stimulates nuclear export of dsRNA binding proteins eEF1A and tRNA.
Human, Mouse, Rat
Recombinant Rabbit Monoclonal Antibody
Recombinant protein within human Exportin-5 aa 1-200.
293, rat epididymis tissue, human lung cancer tissue, human thyroid gland tissue, human breast tissue, mouse testis tissue, K562.
Exp 5 antibody
Exportin 5 antibody
Ran binding protein 21 antibody
Ran-binding protein 21 antibody
Ranbp 21 antibody
Xpo 5 antibody
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
ProA affinity purified
Fig1: Western blot analysis of Exportin-5 on 293 cell using anti-Exportin-5 antibody at 1/2,000 dilution.
Fig2: Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-Exportin-5 antibody. Counter stained with hematoxylin.
Fig3: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Exportin-5 antibody. Counter stained with hematoxylin.
Fig4: Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using anti-Exportin-5 antibody. Counter stained with hematoxylin.
Fig5: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-Exportin-5 antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Exportin-5 antibody. Counter stained with hematoxylin.
Fig7: Flow cytometric analysis of K562 cells with Exportin-5 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
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To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
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