NF-1, also designated CTF, consists of a family of CCAAT box binding proteins that stimulate DNA replication and activate transcription. Analysis of human NF-1 messenger RNA has revealed two forms of the NF-1 protein arising from an alternate splicing of a single NF-1 gene. NF-1 binds its consensus DNA element as a homodimer via an amino-terminal DNA binding domain, and activates transcription through a putatively novel, proline-rich, carboxy terminal transactivation domain. The NF-1 protein has been shown to recognize and bind the adenovirus type 2 promoter and activate transcription of herpes simplex virus thymidine kinase genes. The NF-1 consensus element has been found in the upstream promoter region of myriad eukaryotic genes, including that of Ha-Ras, α-globin, HSP 70, GRP 78, Histone H1, myelin basic protein and in the Xenopus laevis vitellogenin gene promoter.
Human, Mouse, Rat
Mouse Monoclonal Antibody
Recombinant full length protein corresponding to human NFIB/NF1B2.
SH-SY5Y, rat heart tissue, human pancreas tissue, mouse liver tissue.
CCAAT Box Binding Transcription Factor antibody
CCAAT-box-binding transcription factor antibody
Nuclear factor 1 B-type antibody
Nuclear factor 1/B antibody
Nuclear Factor 1B antibody
Nuclear Factor I B antibody
Nuclear factor I/B antibody
TGGCA Binding Protein antibody
TGGCA-binding protein antibody
TRANSCRIPTION FACTOR NFIB antibody
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
ProG affinity purified.
Fig1: Western blot analysis of NFIB/NF1B2 on SH-SY5Y using anti-NFIB/NF1B2 antibody at 1/500 dilution.
Fig2: Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-NFIB/NF1B2 antibody. Counter stained with hematoxylin.
Fig3: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-NFIB/NF1B2 antibody. Counter stained with hematoxylin.
Fig4: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-NFIB/NF1B2 antibody. Counter stained with hematoxylin.
Fig5: Flow cytometric analysis of SH-SY5Y cells with NFIB/NF1B2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody.
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To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
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