Cytochrome P450 Reductase
P450 enzymes constitute a family of monooxygenase enzymes that are involved in the metabolism of a wide array of endogenous and xenobiotic compounds. Several P450 enzymes have been classified by sequence similarities as members of the CYP1A and CYP2A subfamilies. CYPOR, also known as cytochrome P450 reductase and NADPH cytochrome P450 reductase, is a microsomal enzyme responsible for the transfer of electrons from NADPH to cytochrome P450 enzymes during the P450 catalytic cycle. CYPOR is localized to the endoplasmic reticulum, where it is also able to transfer electrons to heme oxygenase and cytochrome b5. CYPOR is structurally related to two separate flavoprotein families, ferredoxin nucleotide reductase (FNR) and flavodoxin. Electron transfer of CYPOR requires the binding of two flavin cofactors, FAD and FMN, to the FNR and flavodoxin domains, respectively.
Human, Mouse, Rat
Recombinant Rabbit Monoclonal Antibody
SK-Br-3, A549, mouse lung tissue lysate, rat brain tissue, human lung cancer tissue, human liver tissue, mouse testis tissue, HepG2.
Cytochrome p450 oxidoreductase antibody
NADPH Cytochrome P450 Reductase antibody
NADPH dependent cytochrome P450 reductase antibody
NADPHcytochrome P450 reductase antibody
P450 (cytochrome) oxidoreductase antibody
P450 Cytochrome Oxidoreductase antibody
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
ProA affinity purified
Fig1: Western blot analysis of Cytochrome P450 Reductase on SK-Br-3 (1) , A549 (2) and mouse lung (3) lysate using anti-Cytochrome P450 Reductase antibody at 1/500 dilution.
Fig2: ICC staining Cytochrome P450 Reductase in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining Cytochrome P450 Reductase in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining Cytochrome P450 Reductase in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Cytochrome P450 Reductase antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Cytochrome P450 Reductase antibody. Counter stained with hematoxylin.
Fig7: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Cytochrome P450 Reductase antibody. Counter stained with hematoxylin.
Fig8: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Cytochrome P450 Reductase antibody. Counter stained with hematoxylin.
Fig9: Flow cytometric analysis of HepG2 cells with Cytochrome P450 Reductase antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
1. Xia C et al. Structural basis for human NADPH-cytochrome P450 oxidoreductase deficiency. Proc Natl Acad Sci USA 108:13486-13491 (2011).
2. Zhao Q et al. Crystal structure of the FMN-binding domain of human cytochrome P450 reductase at 1.93 A resolution. Protein Sci 8:298-306 (1999).
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To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
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