Normal adjustment to changes in blood glucose levels depends on insulin signaling as well as enzymes involved in the regulation of gluconeogenesis. Pathological changes to this process are central to the type 2 diabetes phenotype. Phosphoenolpyruvate carboxykinase (PEPCK) plays an important role in this process by stimulating hepatic glucose production. PEPCK expression increases in response to glucagon and glucocorticoids, while insulin suppresses expression. Modulation of the signals governing PEPCK levels present a potential therapeutic approach to the treatment of insulin resistance and consequently obesity. The cytosolic form of PEPCK, known as PEPCK-C, and the mitochondrial form, known as PEPCK-M, are encoded by two different nuclear genes in mouse, human and chicken.
Recombinant Rabbit Monoclonal Antibody
293T, HepG2, SH-SY5Y, human liver tissue, human kidney tissue.
cytosolic [GTP] antibody
PEP carboxykinase antibody
Phosphoenolpyruvate carboxykinase 1 (soluble) antibody
Phosphoenolpyruvate carboxykinase 1 antibody
Phosphoenolpyruvate carboxykinase antibody
Phosphoenolpyruvate carboxykinase, cytosolic [GTP] antibody
Phosphoenolpyruvate carboxykinase, cytosolic antibody
Phosphoenolpyruvate carboxylase antibody
Phosphopyruvate carboxylase antibody
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
ProA affinity purified
Fig1: ICC staining PCK1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig2: ICC staining PCK1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining PCK1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-PCK1 antibody. Counter stained with hematoxylin.
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PCK1 antibody. Counter stained with hematoxylin.
1. Adams D R et al. Three rare diseases in one sib pair: RAI1, PCK1, GRIN2B mutations associated with Smith-Magenis Syndrome, cytosolic PEPCK deficiency and NMDA receptor glutamate insensitivity. Mol Genet Metab 113:161-170 (2014).
2. Santra S et al. Cytosolic phosphoenolpyruvate carboxykinase deficiency presenting with acute liver failure following gastroenteritis. Mol Genet Metab 118:21-27 (2016).
Publishing research using PCK1? Please let us know so that we can cite the reference in this datasheet.
Choose your product,application and species below,then click "Create".
On the next page you can complete and submit your review.
Leave us a review
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Click here to view the general protocols