RNA-specific adenosine deaminase (ADAR1, DSH, IFI4, p136, DRADA, DSRAD, K88dsRBP) mediates RNA editing by destabilizing double stranded RNA through deamination of adenosine to inosine in structured or double-stranded RNAs. ADAR1 is expressed from an interferon-response promoter and has a Z-DNA/Z-RNA binding domain at its N-terminus. ADAR1 co-localizes with SUMO-1 in a subnucleolar region that is distinct from the fibrillar center, the dense fibrillar component and the granular component. Localization of nuclear ADAR1 is under the influence of a nucleolar localization signal (NoLS) in the middle of ADAR1 and the exporting activity of the nuclear exporter signal (NES) near the N terminus. ADAR1 upregulates nuclear factor 90 (NF90)-mediated gene expression by interacting with NF110, NF90 and NF45. ADAR1 binds short interfering RNA (siRNA), and gene silencing by siRNA is significantly more effective in mouse fibroblasts homozygous for an ADAR1 null mutation than in wild-type cells. ADAR1 may limit the efficacy of siRNA in mammalian cells.
Recombinant Rabbit Monoclonal Antibody
Hela, human lung cancer tissue, human colon cancer tissue, human kidney tissue, human pancreas tissue, SiHa.
136 kDa double-stranded RNA-binding protein antibody
136kDa double stranded RNA binding protein antibody
Adar 1 antibody
Adenosine deaminase acting on RNA 1 A antibody
Adenosine deaminase RNA specific 1 antibody
Adenosine deaminase RNA specific antibody
Adenosine deaminase that act on RNA antibody
Double stranded RNA specific adenosine deaminase antibody
Double-stranded RNA-specific adenosine deaminase antibody
Double-stranded RNA-specific editase Adar antibody
dsRNA adenosine deaminase antibody
EC 3.5.4.- antibody
IFI 4 antibody
Ifi4 protein antibody
Interferon induced protein 4 antibody
Interferon inducible protein 4 antibody
Interferon-inducible protein 4 antibody
Pre-mRNA adenosine deaminase antibody
RNA adenosine deaminase 1 antibody
RNA-editing deaminase 1 antibody
RNA-editing enzyme 1 antibody
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
ProA affinity purified
Fig1: Western blot analysis of ADAR on SiHa cell using anti-ADAR antibody at 1/500 dilution.
Fig2: ICC staining ADAR in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-ADAR antibody. Counter stained with hematoxylin.
Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ADAR antibody. Counter stained with hematoxylin.
Fig5: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-ADAR antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-ADAR antibody. Counter stained with hematoxylin.
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