The translation of proteins from eukaryotic mRNA is initiated by the multisubunit complex eIF-4F, which associates with the mRNA 5' cap structure. eIF-4E, a component of eIF-4F, is responsible for binding to the 5' cap structure and for the assembly of the eIF-4F complex. The regulatory protein 4E-BP1, also referred to as PHAS-I, inhibits eIF-4E function. Phosphorylation of 4E-BP1 by S6 kinase p70, MAP kinases or PKCs causes the disassociation of 4E-BP1 from eIF-4E, promoting translation. A protein that is functionally related to 4E-BP1, designated 4E-BP2, also associates with eIF-4E.
Human, Mouse, Rat
Rabbit Polyclonal Antibody
Mouse placenta tissue, PC-3M, 293T, rat esophagus tissue, human tonsil tissue, human pancreas tissue, mouse small intestine tissue.
BP 1 antibody
eIF4E binding protein 1 antibody
eIF4E-binding protein 1 antibody
Eukaryotic translation initiation factor 4E-binding protein 1 antibody
Phosphorylated heat- and acid-stable protein regulated by insulin 1 antibody
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Protein affinity purified.
Fig1: Western blot analysis of 4E-BP1 on different lysates using anti-4E-BP1 antibody at 1/500 dilution.
Lane2: Mouse placenta tissue
Fig2: ICC staining 4E-BP1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: Immunohistochemical analysis of paraffin-embedded rat esophagus tissue using anti-4E-BP1 antibody. Counter stained with hematoxylin.
Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-4E-BP1 antibody. Counter stained with hematoxylin.
Fig5: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-4E-BP1 antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-4E-BP1 antibody. Counter stained with hematoxylin.
Fig7: Flow cytometric analysis of PC-3M cells with 4E-BP1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
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To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
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