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Product code: R1510-20

  • Overview

  • Product name Nestin
  • Molecular Wt 177 kDa(Predicted band size)
  • Description Nestin is a major intermediate filament (IF) protein of embryonic central nervous system progenitor cells. It is also a component of the dynamic IF network during muscle development, where it polymerizes with Desmin and Vimentin. Nestin co-assembles with Vimentin or a-internexin and forms heterodimer coiled-coil molecules which then further assemble into 10 nml IFs. Deletion of the IF consensus rod domain in nestin alters nestin localization in CNS precursor cells and radial glial cells in vivo. Nestin is a marker for neuroepithelial stem cells, glioma cells and tumor endothelial cells during rapid growth. During axon elongation of differen-tiation neurons, nestin localizes to the growth cones and may play a role in growth cone guidance. In the rat adrenal gland, nestin is expressed by the zona fasciculata and the zona reticularis. Nestin is also expressed by dermatomal cells and by myoblasts during the earliest stages of myogenesis.
  • Species reactivity Human, Mouse
  • Tested applications ICC
  • Antibody type Rabbit Polyclonal Antibody
  • Immunogen Peptide
  • Positive control F9, Hela, N2A, human breast tissue, mouse brain tissue.
  • Conjugate Non-conjugated
  • Alternative names ESTM 46 antibody
    FLJ 21841 antibody
    FLJ21841 antibody
    Intermediate filament protein antibody
    Nbla00170 antibody nes antibody
    NEST_HUMAN antibody Nestin antibody
  • Properties

  • Form Liquid
  • Concentration 1 mg/mL.
  • Storage instructions Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
  • Buffer 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
  • Isotype IgG
  • Purity Peptide affinity purified
  • Subcellular location Cytoskeleton, extracellular region or secreted.
  • Database links Q6P5H2
  • Applications

    ICC: 1:50-1:200

    IHC: 1:50-1:200

    FC: 1:50-1:100

    WB: 1:500

  • Publishing research using Nestin? Please let us know so that we can cite the reference in this datasheet.

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Specific Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

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