IFN-induced antiviral protein which disrupts intracellular cholesterol homeostasis. Inhibits the entry of viruses to the host cell cytoplasm by preventing viral fusion with cholesterol depleted endosomes. May inactivate new enveloped viruses which buds out of the infected cell, by letting them go out with a cholesterol depleted membrane. Active against multiple viruses, including influenza A virus, SARS coronavirus (SARS-CoV), Marburg virus (MARV) and Ebola virus (EBOV), Dengue virus (DNV), West Nile virus (WNV), human immunodeficiency virus type 1 (HIV-1) and vesicular stomatitis virus (VSV). Can inhibit: influenza virus hemagglutinin protein-mediated viral entry, MARV and EBOV GP1,2-mediated viral entry, SARS-CoV S protein-mediated viral entry and VSV G protein-mediated viral entry. Plays a critical role in the structural stability and function of vacuolar ATPase (v-ATPase). Establishes physical contact with the v-ATPase of endosomes which is critical for proper clathrin localization and is also required for the function of the v-ATPase to lower the pH in phagocytic endosomes thus establishing an antiviral state.
Recombinant Rabbit Monoclonal Antibody
Hela, HepG2, HUVEC, MCF-7, PC-3M, human liver cancer tissue, human colon cancer tissue.
1 8U antibody
Interferon induced transmembrane protein 3 (1 8U) antibody
Interferon Induced Transmembrane Protein 3 antibody
Interferon inducible antibody
Interferon inducible protein 1 8U antibody
Interferon Inducible Protein 15 antibody
Interferon Inducible Protein Homolog antibody
Interferon-induced transmembrane protein 3 antibody
Interferon-inducible protein 1-8U antibody
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
ProA affinity purified
Cell membrane. Lysosome.
Fig1: Western blot analysis of Fragilis on different cell lysates using anti-Fragilis antibody at 1/500 dilution. Positive control:
Lane 1: Hela
Lane 2: HepG2
Lane 3: Hela
Fig2: ICC staining Fragilis in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining Fragilis in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining Fragilis in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Fragilis antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Fragilis antibody. Counter stained with hematoxylin.
Fig7: Flow cytometric analysis of MCF-7 cells with Fragilis antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
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To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
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