Myosin Light Chain 2
Encoded by the MYL2 gene, myosin regulatory light chain 2, ventricular/cardiac muscle isoform, also designated MLC-2 or MLC2v, is part of a hexameric complex of two heavy chains and four light chains predominantly expressed in adult cardiac ventricle muscle. Myosin regulatory light chain 2 binds calcium and has been shown to be a useful molecular marker for cardiac chamber specification. The co-expression of myosin regulatory light chain 7 (MYL7) and myosin regulatory light chain 2 in the outflow tract and atrioventricular canal, together with the single expression in the atrial (MYL7) and ventricular (MYL2) myocardium, permits the delineation of their boundaries. At the amino acid level there is 96% homology between the human and mouse myosin regulatory light chain sequences. Mutations in MYL2 are correlated with mid-left ventricular chamber type hypertrophic cardiomyopathy (MVC2) as well as familial hypertrophic cardiomyopathy type 10 (CMH10).
Human, Mouse, Rat
Rabbit Polyclonal Antibody
Mouse skeletal muscle tissue lysate, human skeletal muscle tissue lysate, mouse heart tissue, rat heart tissue, A549, Hela, C2C12.
Cardiac myosin light chain-2 antibody
Cardiac ventricular myosin light chain 2 antibody
MLC 2v antibody
MYL 2 antibody
Myosin light chain 2 regulatory cardiac slow antibody
Myosin light polypeptide 2 regulatory cardiac slow antibody
Myosin regulatory light chain 2 antibody
Myosin regulatory light chain 2 ventricular/cardiac muscle isoform antibody
Regulatory light chain of myosin antibody
RLC of myosin antibody
Slow cardiac myosin regulatory light chain 2 antibody
ventricular/cardiac muscle isoform antibody
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.
Peptide affinity purified
Fig1: Western blot analysis of Myosin Light Chain 2 on different lysates using anti-Myosin Light Chain 2 antibody at 1/500 dilution. Positive control： Lane1: Mouse skeletal muscle Lane2: Human skeletal muscle Lane3: Mouse heart Lane4: Rat heart
Fig2: ICC staining Myosin Light Chain 2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining Myosin Light Chain 2 in C2C12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Myosin Light Chain 2 antibody. Counter stained with hematoxylin.
Fig5: Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-Myosin Light Chain 2 antibody. Counter stained with hematoxylin.
Fig6: Flow cytometric analysis of Hela cells with Myosin Light Chain 2 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.
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