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GCLM

Product code: ET1705-87


  • Overview

  • Product name GCLM
  • Description Gamma-glutamylcysteine synthetase (γ-GCS) is the rate limiting enzyme for glutathione (L-gamma-glutamyl-L-cysteinylglycine, GSH) synthesis. GSH is ubiquitous in mammalian cells as a vital intra- and extracellular protective antioxidant. γ-GCS is a heterodimer of a heavy catalytic subunit and a light regulatory subunit that is responsive to inflammation, phenolic antioxidants, heat shock, oxidants and cytokines. The human gamma-GCS gene encoding the 367 amino acid catalytic subunit maps to chromosome 6p12. The human γ-GCS gene encoding the regulatory subunit maps to chromosome 1p22-p21. The two subunits of γ-GCS form a heterodimeric zinc metalloprotein that gains activity through formation of a reversible disulfide bond.
  • Species reactivity Human, Mouse, Rat
  • Tested applications WB,ICC/IF,IHC,FC,IP
  • Antibody type Recombinant Rabbit Monoclonal Antibody
  • Immunogen Recombinant protein
  • Positive control A431, PC-12, NIH-3T3, Hela, A549,human colon cancer tissue, human pancreas tissue, mouse small intestine tissue.
  • Conjugate Non-conjugated
  • Alternative names Gamma ECS regulatory subunit antibody
    Gamma-ECS regulatory subunit antibody
    Gamma-glutamylcysteine synthetase regulatory subunit antibody
    GCLM antibody
    GCS light chain antibody
    GLCLR antibody
    Glutamate cysteine ligase regulatory subunit antibody
    Glutamatecysteine ligase modifier subunit antibody
    Glutamatecysteine ligase regulatory subunit antibody
    GSC light chain antibody
    GSH0_HUMAN antibody
  • Properties

  • Form Liquid
  • Concentration 1 mg/mL.
  • Storage instructions Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
  • Buffer 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
  • Isotype IgG
  • Purity ProA affinity purified
  • Subcellular location Cytosol.
  • Database links P48507(Human)
    O09172(Mouse)
    P48508(Rat)
  • Applications

    WB: 1:500-1:1,000
    ICC: 1:50-1:200
    IHC: 1:50-1:100
    FC: 1:50-1:100

  • Publishing research using GCLM? Please let us know so that we can cite the reference in this datasheet.

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Specific Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols
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