Steroid hormones function as signaling molecules by diffusing into cells and interacting with specific intracellular receptors to regulate gene expression. This superfamily of receptors includes both steroid and nonsteroid receptors. Like many nonsteroid hormone receptors, PXR (Pregnane X Receptor) binds as a heterodimer with RXR to a DNA sequence typical of a nonsteroid hormone receptor; however, PXR is activated by several steroids, such as naturally occurring pregnanes and synthetic glucocorticoids and anti- glucocorticoids. PXR exists as two alternatively spliced isoforms, PXR.1 and PXR.2. PXR is thought to define a novel steroid hormone signaling pathway that may account for some of the effects of synthetic glucocorticoids and antiglucocorticoids that are not mediated through the classical glucocorticoid receptor signaling pathway.
Mouse Monoclonal Antibody
Human NR1I2 recombinant protein, NR1I2-hIgGFc transfected HEK293 cell lysate, Hela, HepG2.
Nuclear receptor subfamily 1 group I member 2 antibody
ONR 1 antibody
Orphan nuclear receptor PAR 1 antibody
Orphan nuclear receptor PAR1 antibody
Orphan nuclear receptor PXR antibody
PAR 1 antibody
PAR 2 antibody
PAR q antibody
pregnane X nuclear receptor variant 2 antibody
Pregnane X receptor antibody
Steroid and xenobiotic receptor antibody
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
ProA affinity purified
Nucleus,intermediate filament cytoskeleton.
Fig1: Western blot analysis of NR1I2 on human NR1I2 recombinant protein using anti-NR1I2 antibody at 1/1,000 dilution.
Fig2: Western blot analysis of NR1I2 on HEK293 (1) and NR1I2-hIgGFc transfected HEK293 (2) cell lysate using anti-NR1I2 antibody at 1/1,000 dilution.
Fig3: ICC staining NR1I2 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4:Flow cytometric analysis of HepG2 cells with NR1I2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
1. Sivertsson L ?et al.?Induced CYP3A4 expression in confluent Huh7 hepatoma cells as a result of decreased cell proliferation and subsequent pregnane X receptor activation.?Mol Pharmacol?83:659-70 (2013).
2. Dou W ?et al.?Chrysin ameliorates chemically induced colitis in the mouse through modulation of a PXR/NF-?B signaling pathway.?J Pharmacol Exp Ther?345:473-82 (2013).
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To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
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