This gene encodes a transcription termination factor that is localized to the nucleolus and plays a critical role in ribosomal gene transcription. The encoded protein mediates the termination of RNA polymerase I transcription by binding to Sal box terminator elements downstream of pre-rRNA coding regions. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. This gene shares the symbol/alias 'TFF1' with another gene, NK2 homeobox 1, also known as thyroid transcription factor 1, which plays a role in the regulation of thyroid-specific gene expression. Multifunctional nucleolar protein that terminates ribosomal gene transcription, mediates replication fork arrest and regulates RNA polymerase I transcription on chromatin. Plays a dual role in rDNA regulation, being involved in both activation and silencing of rDNA transcription. Interaction with BAZ2A/TIP5 recovers DNA-binding activity.
Mouse Monoclonal Antibody
Human TTF1 recombinant protein, TTF1-hIgGFc transfected HEK293 cell lysate, MCF-7, SK-OV-3, Hela, human rectum cancer tissue, human cervical cancer tissue.
Benign chorea antibody
Homeobox protein NK 2 homolog A antibody
Homeobox protein NK-2 homolog A antibody
Homeobox protein Nkx 2.1 antibody
Homeobox protein Nkx-2.1 antibody
Homeobox protein Nkx2.1 antibody
NK 2 antibody
NK 2 homolog A antibody
NK2 homeobox 1 antibody
NK2, drosophila, homolog of, A antibody
NK2.1, mouse, homolog of antibody
Nkx 2 1 antibody
NKX 2.1 antibody
NKX 2A antibody
NKX2 1 antibody
T EBP antibody
Thyroid nuclear factor 1 antibody
Thyroid nuclear factor antibody
Thyroid specific enhancer binding protein antibody
Thyroid transcription factor 1 antibody
Tin man antibody
TITF 1 antibody
TTF 1 antibody
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
ProA affinity purified
WB: 1:500-1:2,000 ICC: 1:50-1:200 IHC: 1:50-1:200 FC: 1:50-1:200
Fig1: Western blot analysis of TTF1 on ?human TTF1 recombinant protein using anti-TTF1 antibody at 1/1,000 dilution.
Fig2: Western blot analysis of TTF1 on HEK293 (1) and TTF1-hIgGFc transfected HEK293 (2) cell lysate using anti-TTF1 antibody at 1/1,000 dilution.
Fig3: ICC staining TTF1 (green) and Actin filaments (red) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4:ICC staining TTF1 (green) and Actin filaments (red) in SK-OV-3 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-TTF1 antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-TTF1 antibody. Counter stained with hematoxylin.
Fig7:Flow cytometric analysis of Hela cells with TTF1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
1. Chang HW ?et al.?Characterization of a transgenic mouse model exhibiting spontaneous lung adenocarcinomas with a metastatic phenotype.?PLoS One?12:e0175586 (2017). 2. Wang Y ?et al.?LSD1 co-repressor Rcor2 orchestrates neurogenesis in the developing mouse brain.Nat Commun?7:10481 (2016).
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To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
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