The mGluR proteins (metabotropic Glutamate Receptors) are members of the G-protein-coupled receptor family and are functionally and pharmacologically distinct from the GluR proteins (ionotropic Glutamate Receptors). The eight currently known mGluR proteins are mediated by two G-proteins with opposing regulation of adenylate cyclase pathways. The activities of mGluR1 and mGluR5 are mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system and generates a calcium-activated chloride current. The remainder of the eight sub-types of mGluR have an activity mediated by a G-protein that inhibits adenylate cyclase activity. mGluR-3, which may interact with GRASP, acts as a receptor for glutamate.
Mouse Monoclonal Antibody
Human GRM3 recombinant protein, GRM3-hIgGFc transfected HEK293 cell lysate, Hela, human cervical cancer tissue, SH-SY5Y.
Glutamate metabotropic receptor 3 antibody
Glutamate receptor metabotropic 3 antibody
Metabotropic glutamate receptor 3 antibody
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
ProA affinity purified
WB: 1:500-1:2,000 ICC: 1:50-1:200 IHC: 1:50-1:200 FC: 1:50-1:100
Fig1: Western blot analysis of GRM3 on human GRM3 recombinant protein using anti-GRM3 antibody at 1/1,000 dilution.
Fig2: Western blot analysis of GRM3 on HEK293 (1) and GRM3-hIgGFc transfected HEK293 (2) cell lysate using anti-GRM3 antibody at 1/1,000 dilution.
Fig3: ICC staining GRM3 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-GRM3 antibody. Counter stained with hematoxylin.
Fig5: Flow cytometric analysis of SH-SY5Y cells with GRM3 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
1. Kinoshita A et al. Effect of metabotropic glutamate receptor-3 variants on prefrontal brain activity in schizophrenia: An imaging genetics study using multi-channel near-infrared spectroscopy. Prog Neuropsychopharmacol Biol Psychiatry 62:14-21 (2015). 2. Guo J et al. mGluR3 promotes proliferation of human embryonic cortical neural progenitor cells by activating ERK1/2 and JNK2 signaling pathway in vitro. Cell Mol Biol (Noisy-le-grand) 60(2):42-9 (2014).
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