The immunosuppressant cyclosporin A (CsA) forms a trimolecular complex with cyclophilin and calcineurins to inhibit calcineurin phosphatase activity. Cyclophilins are conserved, ubiquitous and abundant cytosolic peptidyl-prolyl cis-trans isomerases that accelerate the isomerization of XaaPro peptide bonds and the refolding of proteins. Human cyclophilin A (CyPA), an intracellular protein of 165 amino acids, is the target of the CsA and is encoded by a single unique gene conserved from yeast to humans. CyPA is known for its involvement in T cell differentiation and proliferation and is highly expressed in brain. CyPA is incorporated into the virion of the type 1 human immunodeficiency virus (HIV-1) via a direct interaction with the capsid domain of the viral Gag polyprotein and is crucial for efficient viral replication. Cyclophilin B (CyPB) is a member of the cyclophilin family with specific N- and C-terminal extensions. Unlike CyPA, CyPB has a signal sequence leading to its translocation in the endoplasmic reticulum. CyPB is secreted in biological fluids such as blood or milk and binds to a specific receptor present on the human lymphoblastic cell line Jurkat and on human peripheral blood lymphocytes (9,10).
Human, Mouse, Rat
Recombinant Rabbit Monoclonal Antibody
Hela, HepG2, A431, human breast carcinoma tissue, human lung tissue, human pancreas tissue, mouse lung tissue.
Cyclophilin A antibody
Cyclosporin A binding protein antibody
Cyclosporin A-binding protein antibody
Epididymis secretory sperm binding protein Li 69p antibody
HEL S 69p antibody
Peptidyl prolyl cis trans isomerase A antibody
Peptidyl-prolyl cis-trans isomerase A antibody
Peptidylprolyl isomerase A (cyclophilin A) antibody
Peptidylprolyl isomerase A antibody
PPIA protein antibody
PPIase A antibody
Rotamase A antibody
T cell cyclophilin antibody
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
ProA affinity purified
WB: 1:1,000-1:5,000 IHC: 1:50-1:200
Fig1: Western blot analysis of Cyclophilin A on different cells lysates using anti-Cyclophilin A antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: A431 Lane 3: HepG2
Fig2: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Cyclophilin A antibody. Counter stained with hematoxylin.
Fig3: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Cyclophilin A antibody. Counter stained with hematoxylin.
Fig4: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Cyclophilin A antibody. Counter stained with hematoxylin.
Fig5: Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-Cyclophilin A antibody. Counter stained with hematoxylin.
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