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Cardiac Troponin T [SC64-03]

Product code: ET1610-51

  • Overview

  • Product name Cardiac Troponin T
  • Molecular Wt 36 kDa
  • Description Actin is a highly conserved protein that is expressed in all eukaryotic cells. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. Myosin is a hexamer of two heavy chains (MHC) and four light chains (MLC) that interacts with Actin to generate the force for diverse cellular movements, including cytokinesis, phagocytosis and muscle contraction. Troponin facilitates the interaction between Actin and Myosin by binding to calcium. Troponin is made up of at least two subunits, which are divergent in cardiac muscle, fast skeletal muscle and slow skeletal muscle. Structures of skeletal muscle troponin are composed of Troponin C (the sensor), Troponin I (the regulator) and Troponin T (the link to the muscle thin filament). Troponin C is dumbbell-shaped and has a hydrophobic pocket that increases the contractile force of muscle fibers. Troponin C has two isoforms: fast and slow. Fast Troponin C has two calcium binding sites, while slow/cardiac Troponin C has a single calcium binding site.
  • Species reactivity Human, Mouse, Rat
  • Tested applications WB,IHC
  • Antibody type Recombinant Rabbit Monoclonal Antibody
  • Immunogen recombinant protein
  • Positive control Mouse heart tissue.
  • Conjugate Non-conjugated
  • Clone number SC64-03
  • Alternative names Cardiac muscle troponin T antibody
    Cardiomyopathy dilated 1D (autosomal dominant) antibody
    Cardiomyopathy hypertrophic 2 antibody
    CMD1D antibody
    CMH2 antibody
    CMPD2 antibody
    cTnT antibody
    LVNC6 antibody
    MGC3889 antibody
    OTTHUMP00000033864 antibody
    OTTHUMP00000033865 antibody
    OTTHUMP00000033866 antibody
    OTTHUMP00000033867 antibody
    OTTHUMP00000033870 antibody
    OTTHUMP00000218095 antibody
    RCM3 antibody
    TNNT 2 antibody
    TNNT2 antibody
    TNNT2_HUMAN antibody
    TnTc antibody
    Troponin T cardiac muscle antibody
    Troponin T type 2 (cardiac) antibody
    Troponin T type 2 cardiac antibody
    Troponin T, cardiac muscle antibody
    Troponin T2 cardiac antibody
  • Properties

  • Form Liquid
  • Concentration 1 mg/mL.
  • Storage instructions Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
  • Buffer 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
  • Isotype IgG
  • Purity ProA affinity purified
  • Subcellular location Cytoplasm, filament.
  • Database links P45379(Human)
  • Applications

    WB: 1:1,000-1:2,000
    IHC: 1:100-1:500

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Specific Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

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