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Phospho-PKA R2 (S99) [SC54-04]

Product code: ET1610-29


  • Overview

  • Product name Phospho-PKA R2 (S99)
  • Molecular Wt 51 kDa
  • Description The second messenger cyclic AMP (cAMP) mediates diverse cellular responses to external signals such as proliferation, ion transport, regulation of metabolism and gene transcription by activation of the cAMP-dependent protein kinase (cAPK or PKA). Activation of PKA occurs when cAMP binds to the two regulatory subunits of the tetrameric PKA holoenzyme resulting in release of active catalytic subunits. Three catalytic (C) subunits have been identified, designated Cα, Cβ and Cγ, that each represent specific gene products. Cα and Cβ are closely related (93% amino acid sequence similarity), whereas Cγ displays 83% and 79% similarity to Cα and Cβ, respectively. Activation of transcription upon elevation of cAMP levels results from translocation of PKA to the nucleus where it phosphorylates the transcription factor cAMP response element binding protein (CREB) on serine 133 which in turn leads to TFIIB binding to TATA-box-binding protein TBP1, thus linking phospho-CREB to the pol II transcription initiation complex.
  • Species reactivity Human, Mouse, Rat, Pig
  • Tested applications WB,ICC/IF,IHC,IP
  • Antibody type Recombinant Rabbit Monoclonal Antibody
  • Immunogen recombinant protein
  • Positive control MCF-7, Hela, human spleen tissue, mouse testis tissue, mouse heart tissue.
  • Conjugate Non-conjugated
  • Clone number SC54-04
  • Alternative names cAMP dependent protein kinase regulatory subunit alpha 2 antibody
    cAMP dependent protein kinase regulatory subunit RII alpha antibody
    cAMP dependent protein kinase type II alpha regulatory chain antibody
    cAMP dependent protein kinase type II alpha regulatory subunit antibody
    cAMP-dependent protein kinase type II-alpha regulatory subunit antibody
    KAP2 antibody
    KAP2_HUMAN antibody
    MGC3606 antibody
    PKR 2 antibody
    PKR2 antibody
    PRKA R2 antibody
    PRKAR 2 antibody
    PRKAR2 antibody
    PRKAR2A antibody
    Protein kinase A RII alpha subunit antibody
    Protein kinase cAMP dependent regulatory type II alpha antibody
  • Properties

  • Form Liquid
  • Concentration 1 mg/mL.
  • Storage instructions Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
  • Buffer 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
  • Isotype IgG
  • Purity ProA affinity purified
  • Subcellular location Cytoplasm, Cell membrane.
  • Database links P13861(Human)
    P12367(Mouse)
    P12368(Rat)
  • Applications

    WB: 1:500
    ICC: 1:50-1:200
    IHC: 1:50-1:200

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Specific Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

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