Sodium Potassium ATPase
The ubiquitously expressed sodium/potassium-ATPase (Na+/K+-ATPase) exists as an oligomeric plasma membrane complex that couples the hydrolysis of one molecule of ATP to the importation of three Na+ ions and two K+ ions against their respective electrochemical gradients. As a member of the P-type family of ion motives, Na+/K+-ATPase plays a critical role in maintaining cellular volume, resting membrane potential and Na+-coupled solute transport. Multiple isoforms of three subunits, α, β and γ, comprise the Na+/K+-ATPase oligomer. The α subunit contains the binding sites for ATP and the cations; the glycosylated β subunit ensures correct folding and membrane insertion of the α subunits. The small γ subunit co-localizes with the α subunit in nephron segments, where it increases the affinity of Na+/K+-ATPase for ATP. The β subunit, but not the γ subunit, is essential for normal activity of Na+/K+-ATPase.
Human, Mouse, Rat
Recombinant Rabbit Monoclonal Antibody
A549, MCF-7, Hela, HepG2, human liver tissue, mouse liver tissue, mouse kidney tissue, human kidney tissue.
ATPase Na+/K+ transporting alpha antibody
ATPase Na+/K+ transporting alpha 1 polypeptide antibody
ATPase Na+/K+ transporting alpha 4 polypeptide antibody
ATPase Na+/K+ transporting beta 1 polypeptide antibody
ATPase, Na+/K+ transporting, alpha polypeptide-like 2 antibody
ATPase, Na+/K+ transporting, beta 1 polypeptide antibody
Beta 1-subunit of Na(+),K(+)-ATPase antibody
Na(+)/K(+) ATPase alpha-1 subunit antibody
Na(+)/K(+) ATPase alpha-4 subunit antibody
Na+, K+ ATPase alpha subunit antibody
Na+/K+ ATPase 1 antibody
Na+/K+ ATPase 4 antibody
Na+/K+ ATPase, alpha-D polypeptide antibody
Na, K-ATPase beta-1 polypeptide antibody
Na, K-ATPase, alpha-A catalytic polypeptide antibody
Na,K-ATPase catalytic subunit alpha-A protein antibody
Na,K-ATPase subunit alpha-C antibody
polypeptide-like 2 antibody
Sodium pump 1 antibody
sodium pump 4 antibody
Sodium pump subunit alpha-1 antibody
sodium pump subunit alpha-4 antibody
sodium pump subunit beta-1 antibody
sodium-potassium ATPase catalytic subunit alpha-1 antibody
sodium-potassium ATPase catalytic subunit alpha-4 antibody
sodium-potassium ATPase subunit beta 1 (non-catalytic) antibody
sodium-potassium ATPase, alpha 4 polypeptide antibody
sodium-potassium-ATPase, alpha 1 polypeptide antibody
Sodium/potassium transporting ATPase alpha 1 chain antibody
Sodium/potassium transporting ATPase subunit beta 1 antibody
sodium/potassium-dependent ATPase beta-1 subunit antibody
sodium/potassium-transporting ATPase alpha-4 chain antibody
sodium/potassium-transporting ATPase beta-1 chain antibody
Sodium/potassium-transporting ATPase subunit alpha-1 antibody
sodium/potassium-transporting ATPase subunit alpha-4 antibody
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
ProA affinity purified
Cell membrane, Melanosome.
Fig1: Western blot analysis of Sodium Potassium ATPase on A549 cell lysates using anti-Sodium Potassium ATPase antibody at 1/1,000 dilution.
Fig2: ICC staining Sodium Potassium ATPase in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining Sodium Potassium ATPase in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Sodium Potassium ATPase antibody. Counter stained with hematoxylin.
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Sodium Potassium ATPase antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Sodium Potassium ATPase antibody. Counter stained with hematoxylin.
Fig7: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Sodium Potassium ATPase antibody. Counter stained with hematoxylin.
Fig8: Flow cytometric analysis of Hela cells with Sodium Potassium ATPase antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
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To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
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