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Vimentin [D4-B11]

Product code: EM0401

  • Overview

  • Product name Vimentin
  • Molecular Wt 54 kDa
  • Description Vimentin plays a significant role in supporting and anchoring the position of the organelles in the cytosol. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. In essence, vimentin is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. Vimentin has been shown to eliminate toxic proteins in JUNQ and IPOD inclusion bodies in asymmetric division of asymmetric division of mammalian cell lines. It has been used as a sarcoma tumor marker to identify mesenchyme. Methylation of the vimentin gene has been established as a biomarker of colon cancer and this is being utilized in the development of fecal tests for colon cancer. High levels of DNA methylation in the promotor region have also been associated with markedly decreased survival in hormone positive breast cancers.
  • Species reactivity Human,mouse,rat
  • Tested applications WB,ICC,IHC,FC
  • Antibody type Mouse Monoclonal Antibody
  • Immunogen peptide
  • Positive control A549, Hela, NIH/3T3, mouse embryonic stem cell human lung?tissue, human colon carcinoma tissue, human stomach carcinoma tissue, mouse lung tissue
  • Conjugate Non-conjugated
  • Clone number D4-B11
  • Alternative names CTRCT30 antibody
    Epididymis luminal protein 113 antibody
    FLJ36605 antibody
    HEL113 antibody
    VIM antibody
    VIME_HUMAN antibody
    Vimentin antibody
  • Properties

  • Form Liquid
  • Concentration 2 mg/mL.
  • Storage instructions Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
  • Buffer 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
  • Isotype IgG1
  • Purity ProA affinity purified
  • Subcellular location Cytoplasm
  • Database links P08670
  • Applications

    WB: 1:2,000-1:5,000
    ICC: 1:200
    IHC: 1:200
    FC: 1:50-1:100

  • Publishing research using Vimentin? Please let us know so that we can cite the reference in this datasheet.

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Specific Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

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