Vimentin plays a significant role in supporting and anchoring the position of the organelles in the cytosol. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. In essence, vimentin is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. Vimentin has been shown to eliminate toxic proteins in JUNQ and IPOD inclusion bodies in asymmetric division of asymmetric division of mammalian cell lines. It has been used as a sarcoma tumor marker to identify mesenchyme. Methylation of the vimentin gene has been established as a biomarker of colon cancer and this is being utilized in the development of fecal tests for colon cancer. High levels of DNA methylation in the promotor region have also been associated with markedly decreased survival in hormone positive breast cancers.
Mouse Monoclonal Antibody
A549, Hela, NIH/3T3, mouse embryonic stem cell human lung?tissue, human colon carcinoma tissue, human stomach carcinoma tissue, mouse lung tissue
Epididymis luminal protein 113 antibody
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
ProA affinity purified
Fig1: Western blot analysis of Vimentin on different cell lysates using anti- Vimentin antibody at 1/2000 dilution. Positive?control:
Lane 1: A549
Lane 2: Hela
Lane 3: NIH/3T3
Lane 4: Mouse embryonic stem cell
Fig2: ICC staining vimentin in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: Immunohistochemical analysis of paraffin-embedded human lung tissue using vimentin antibody. Counter stained with hematoxylin.
Fig4: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using vimentin antibody. Counter stained with hematoxylin.
Fig5: Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue using vimentin antibody. Counter stained with hematoxylin.
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To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
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