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Histone H3.3 [ST50-08]

Product code: ET1609-59

  • Overview

  • Product name Histone H3.3
  • Molecular Wt 15 kDa
  • Description Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene, that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.
  • Species reactivity Human, Mouse, Rat
  • Tested applications WB,ICC/IF,IHC,CHIP
  • Antibody type Recombinant Rabbit Monoclonal Antibody
  • Immunogen recombinant protein
  • Positive control BT-20, NIH/3T3, human breast carcinoma tissue, mouse brain tissue, mouse colon tissue, mouse stomach tissue.
  • Conjugate Non-conjugated
  • Clone number ST50-08
  • Alternative names H3 histone family 3A antibody
    H3 histone family 3B antibody
    H3 histone, family 3B (H3.3B) antibody
    H3.3 antibody
    H3.3A antibody
    H3.3B antibody
    H33_HUMAN antibody
    H3F3 antibody
    H3F3A antibody
    H3f3b antibody
    Histone H3.3 antibody
    Histone H3.3Q antibody
    Histone H3.A antibody
    Histone H3.B antibody
    MGC87782 antibody
    MGC87783 antibody
  • Properties

  • Form Liquid
  • Concentration 1 mg/mL.
  • Storage instructions Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
  • Buffer 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
  • Isotype IgG
  • Purity ProA affinity purified
  • Subcellular location Nucleus, Chromosome.
  • Database links P84243(Human)
  • Applications

    WB: 1:1,000-1:2,000
    ICC: 1:50-1:200
    IHC: 1:50-1:200

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Specific Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

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