Mitogen-activated protein kinase 14, also called p38-α, is an enzyme that in humans is encoded by the MAPK14 gene. The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress-related transcription and cell cycle regulation, as well as in genotoxic stress response.
Mouse Monoclonal Antibody
HepG2, A431, NIH/3T3, Hela, Jurkat, 293T, mouse placenta tissue，human colon carcinoma tissue，human stomach carcinoma tissue,mouse stomach tissue.
CSAID Binding Protein 1 antibody CSAID binding protein antibody CSAID-binding protein antibody Csaids binding protein antibody CSBP 1 antibody CSBP 2 antibody CSBP antibody CSBP1 antibody CSBP2 antibody CSPB1 antibody Cytokine suppressive anti-inflammatory drug-binding protein antibody EXIP antibody MAP kinase 14 antibody MAP kinase MXI2 antibody MAP kinase p38 alpha antibody MAPK 14 antibody MAPK14 antibody MAX interacting protein 2 antibody MAX-interacting protein 2 antibody Mitogen Activated Protein Kinase 14 antibody Mitogen activated protein kinase p38 alpha antibody Mitogen-activated protein kinase 14 antibody Mitogen-activated protein kinase p38 alpha antibody MK14_HUMAN antibody Mxi 2 antibody MXI2 antibody p38 ALPHA antibody p38 antibody p38 MAP kinase antibody p38 MAPK antibody p38 mitogen activated protein kinase antibody p38ALPHA antibody p38alpha Exip antibody PRKM14 antibody PRKM15 antibody RK antibody SAPK2A antibody
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
ProA affinity purified
WB: 1:500 ICC: 1:100-1:200 IHC: 1:100-1:200
Fig1: Western blot analysis of MAPK14 on different cell lysates using anti-MAPK14 antibody at 1/500 dilution. Positive control: Lane 1: A431 Lane 2: HepG2 Lane 3: Hela Lane 4: NIH/3T3 Lane 5: Jurkat Lane 6: 293T Lane 7: mouse placenta
Fig2: ICC staining of MAPK14 in Hela cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-MAPK14 antibody. Counter stained with hematoxylin.
Fig4: Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue using anti-MAPK14 antibody. Counter stained with hematoxylin.
Fig5: Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-MAPK14 antibody. Counter stained with hematoxylin.
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