beta Ⅱ Tubulin
Tubulin is a major cytoskeleton component that has five distinct forms, designated a, b, g, d and e tubulin. a and b tubulins form heterodimers which multimerize to form a microtubule filament. Multiple b Tubulin isoforms (b1, b2, b3, b4, b5, b6 and b8) have been characterized and are expressed in mammalian tissues. b1 and b4 are present throughout the cytosol, b2 is present in the nuclei and nucleoplasm, and b3 is a neuron-specific cytoskeletal protein. g Tubulin forms the gammasome, which is required for nucleating microtubule filaments at the centrosome. Both d Tubulin and e Tubulin are associated with the centrosome. d Tubulin is a homolog of the Chlamydomonas d Tubulin Uni3 and is found in association with the centrioles, whereas e Tubulin localizes to the pericentriolar material. e Tubulin exhibits a cell cycle-specific pattern of localization; first associating with only the older of the centrosomes in a newly duplicated pair, and later associating with both centrosomes.
Human, Mouse, Rat
Recombinant Rabbit Monoclonal Antibody
N2A, human kidney tissue, mouse liver tissue, mouse brain tissue, mouse kidney tissue.
bA506K6.1 antibody Beta2 antibody class II beta tubulin isotype antibody class IIa beta-tubulin antibody class IIb beta-tubulin antibody Class IVb beta tubulin antibody dJ40E16.7 antibody DKFZp566F223 antibody FLJ98847 antibody M(beta)2 antibody MGC8685 antibody OTTHUMP00000015956 antibody OTTHUMP00000015964 antibody TBB4B_HUMAN antibody TUBB 2 antibody TUBB 2A antibody TUBB 2C antibody TUBB antibody TUBB PARALOG antibody TUBB2 antibody TUBB2A antibody TUBB2B antibody TUBB2C antibody Tubb4b antibody Tubulin beta 2 antibody Tubulin beta 2 chain antibody Tubulin beta 2A antibody Tubulin beta 2A chain antibody Tubulin beta 2B antibody Tubulin beta 2B chain antibody Tubulin beta 2C antibody Tubulin beta polypeptide antibody Tubulin beta polypeptide 2 antibody Tubulin beta polypeptide paralog antibody Tubulin beta-2 chain antibody Tubulin beta-2C chain antibody Tubulin beta-4B chain antibody tubulin, beta 2A class IIa antibody tubulin, beta 2B class IIb antibody tubulin, beta 4B class IVb antibody Tubulin, beta, class IVB antibody Tubulin, beta-4B antibody
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
ProA affinity purified
WB: 1:1,000-1:5,000 ICC: 1:50-1:200 IHC: 1:50-1:200
Fig1: Western blot analysis of beta Ⅱ Tubulin on different lysates using anti-beta Ⅱ Tubulin antibody at 1/1,000 dilution. Positive control: Lane 1: Mouse brain Lane 2: Mouse kidney
Fig2: ICC staining beta Ⅱ Tubulin in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-beta Ⅱ Tubulin antibody. Counter stained with hematoxylin.
Fig4: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-beta Ⅱ Tubulin antibody. Counter stained with hematoxylin.
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To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
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