Location: Home >Product >Product Detail

Phospho-JNK1/2/3(T183+T183+T221) [ST500]

Product code: ET1609-42


  • Overview

  • Product name Phospho-JNK1/2/3(T183+T183+T221)
  • Molecular Wt 54/46 kDa
  • Description JNKs (c-Jun N-terminal kinases) belong to a family of MAP kinases that are involved in a variety of cellular processes, including transcriptional regulation and cellular proliferation, differentiation and development. JNK2 (c-Jun N-terminal kinase 2) and JNK3 (c-Jun N-terminal kinase 3) are 424 and 464 amino acid proteins, respectively, that each contain one protein kinase domain and use magnesium as a cofactor to catalyze the phosphorylation of target proteins, thereby playing a role in a variety of events throughout the cell. Both JNK2 and JNK3 exist as multiple alternatively spliced isoforms and are subject to post-translational phosphorylation on Thr 183 and Thr 221, respectively, an event which activates JNK2/JNK3 enzymatic activity. Defects in the gene encoding JNK3 are a cause of epileptic encephalopathy of the Lennox-Gastaut type, a group of epileptic disorders characterized by severe psychomotor delay and seizures.
  • Species reactivity Human, Mouse, (Predicted: Rat)
  • Tested applications WB,ICC/IF,IHC-P,IP,FC
  • Antibody type Recombinant Rabbit Monoclonal Antibody
  • Immunogen Synthetic phospho-peptide corresponding to residues surrounding Thr183 + Thr183 + Thr221 of human JNK1/2/3
  • Positive control NIH/3T3 cell lysate treated with Anisomycin, Hela, NIH/3T3, HUVEC, human liver tissue, human uterus tissue, mouse heart tissue.
  • Conjugate Non-conjugated
  • Clone number ST500
  • Alternative names C Jun kinase 2 antibody
    c Jun N terminal kinase 1 antibody
    c Jun N terminal kinase 2 antibody
    c Jun N terminal kinase 3 antibody
    c-Jun N-terminal kinase 1 antibody
    JNK 46 antibody
    JNK 55 antibody
    JNK antibody
    JNK-46 antibody
    JNK1 antibody
    JNK1A2 antibody
    JNK2 antibody
    JNK21B1/2 antibody
    JNK2A antibody
    JNK2ALPHA antibody
    JNK2B antibody
    JNK2BETA antibody
    JNK3 alpha protein kinase antibody
    JNK3 antibody
    JNK3A antibody
    Jun kinase antibody
    JUN N terminal kinase antibody
    MAP kinase 10 antibody
    MAP kinase 8 antibody
    MAP kinase 9 antibody
    MAP kinase p49 3F12 antibody
    MAPK 10 antibody
    MAPK 8 antibody
    MAPK 9 antibody
    MAPK10 antibody
    mapk8 antibody
    MAPK9 antibody
    Mitogen activated protein kinase 10 antibody
    Mitogen activated protein kinase 8 antibody
    Mitogen activated protein kinase 8 isoform JNK1 alpha1 antibody
    Mitogen activated protein kinase 8 isoform JNK1 beta2 antibody
    Mitogen activated protein kinase 9 antibody
    Mitogen-activated protein kinase 8 antibody
    MK08_HUMAN antibody
    p493F12 antibody
    p54a antibody
    p54aSAPK antibody
    p54bSAPK antibody
    PRKM10 antibody
    PRKM8 antibody
    PRKM9 antibody
    SAPK antibody
    SAPK(beta) antibody
    SAPK1 antibody
    SAPK1a antibody
    SAPK1b antibody
    SAPK1c antibody
    Stress activated protein kinase 1 antibody
    Stress activated protein kinase 1a antibody
    Stress activated protein kinase 1b antibody
    Stress activated protein kinase 1c antibody
    Stress activated protein kinase beta antibody
    Stress activated protein kinase JNK1 antibody
    Stress activated protein kinase JNK2 antibody
    Stress activated protein kinase JNK3 antibody
    Stress-activated protein kinase 1c antibody
    Stress-activated protein kinase JNK1 antibody
  • Properties

  • Form Liquid
  • Concentration 1 mg/mL.
  • Storage instructions Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
  • Buffer 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
  • Isotype IgG
  • Purity Protein A affinity purified.
  • Subcellular location Cytoplasm, Nucleus, Membrane, Mitochondrion.
  • Database links SwissProt: P45983 Human
    SwissProt: P45984 Human
    SwissProt: P53779 Human
    SwissProt: Q61831 Mouse
    SwissProt: Q91Y86 Mouse
    SwissProt: Q9WTU6 Mouse
    SwissProt: P49185 Rat
    SwissProt: P49186 Rat
    SwissProt: P49187 Rat
  • Applications

    WB: 1:1,000-1:2,000
    ICC/IF: 1:50-1:200
    IHC-P: 1:50-1:200
    FC: 1:50-1:100
    IP: Use at an assay dependent concentration.

  • Publishing research using Phospho-JNK1/2/3(T183+T183+T221)? Please let us know so that we can cite the reference in this datasheet.

  • Data sorting...

Choose your product,application and species below,then click "Create".

On the next page you can complete and submit your review.

  • * Code NO.
  • * Application
  • *Sample
  • Species Tested
  • Against
  • Choose text
  • Content

Leave us a review

Specific Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols
Add to cart successfully.