An interferon-inducible, RNA-dependent protein serine/threonine kinase, PKR has various designations. Mouse PKR is known as DAI, dsJ, PI kinase, p65, p67 or TIK, whereas human PKR is known as p68 or p69. PKR phosphorylates its substrate, a subunit of protein synthesis initiation factor eIF-2 on Ser 51 to inhibit translation. PKR contains two dsRNA binding motifs required for its activation by dsRNA. Three kinds of regulation of PKR enzymatic activity occur, and these include transcriptional regulation in response to interferon, an autoregulatory mechanism controlling PKR expression at the level of translation, and posttranslational regulation by RNA mediated autophosphorylation. Human PKR contains at least 15 autophosphorylation sites, but only Thr-446 and Thr-451 in the activation loop are critical for its kinase activity. Thr-446 is the in vivo autophosphorylation site of PKR. Mutation of threonine to alanine at position 446 substantially reduces PKR function, and mutant kinase containing Ala-451 is completely inactive.
Recombinant Rabbit Monoclonal Antibody
Synthetic phospho-peptide corresponding to residues surrounding Thr446 of human PKR.
Human kidney tissue.
Double stranded RNA activated protein kinase; antibody
eIF-2A protein kinase 2 antibody
Eukaryotic translation initiation factor 2 alpha kinase 2 antibody
Eukaryotic translation initiation factor 2-alpha kinase 2 antibody
Interferon induced double stranded RNA activated protein kinase antibody
Interferon inducible elF2 alpha kinase antibody
Interferon inducible RNA dependent protein kinase antibody
Interferon-induced, double-stranded RNA-activated protein kinase antibody
Interferon-inducible RNA-dependent protein kinase antibody
P1/eIF-2A protein kinase antibody
P1/eIF2A protein kinase antibody
p68 kinase antibody
Protein kinase interferon inducible double stranded RNA dependent antibody
Protein kinase RNA activated antibody
Protein kinase RNA-activated antibody
Protein phosphatase 1 regulatory subunit 83 antibody
Serine/threonine protein kinase TIK antibody
Tyrosine protein kinase EIF2AK2 antibody
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
ProA affinity purified
Fig1: Western blot analysis of Phospho-PKR(T446) on different lysates using anti-Phospho-PKR(T446) antibody at 1/1,000 dilution. Positive control:
Lane 1: Hela treated with Calyculin A and TNF-alpha whole cell lysates
Lane 2: Untreated Hela whole cell lysates
Fig2: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Phospho-PKR(T446) antibody. Counter stained with hematoxylin.
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