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Phospho-PKR(T446) [SY230]

Product code: ET1607-20

  • Overview

  • Product name Phospho-PKR(T446)
  • Molecular Wt 62 kDa
  • Description An interferon-inducible, RNA-dependent protein serine/threonine kinase, PKR has various designations. Mouse PKR is known as DAI, dsJ, PI kinase, p65, p67 or TIK, whereas human PKR is known as p68 or p69. PKR phosphorylates its substrate, a subunit of protein synthesis initiation factor eIF-2 on Ser 51 to inhibit translation. PKR contains two dsRNA binding motifs required for its activation by dsRNA. Three kinds of regulation of PKR enzymatic activity occur, and these include transcriptional regulation in response to interferon, an autoregulatory mechanism controlling PKR expression at the level of translation, and posttranslational regulation by RNA mediated autophosphorylation. Human PKR contains at least 15 autophosphorylation sites, but only Thr-446 and Thr-451 in the activation loop are critical for its kinase activity. Thr-446 is the in vivo autophosphorylation site of PKR. Mutation of threonine to alanine at position 446 substantially reduces PKR function, and mutant kinase containing Ala-451 is completely inactive.
  • Species reactivity Human
  • Tested applications WB,IHC,IP,CHIP
  • Antibody type Recombinant Rabbit Monoclonal Antibody
  • Immunogen Synthetic phospho-peptide corresponding to residues surrounding Thr446 of human PKR.
  • Positive control Human kidney tissue.
  • Conjugate Non-conjugated
  • Clone number SY230
  • Alternative names Double stranded RNA activated protein kinase; antibody
    E2AK2_HUMAN antibody
    eIF-2A protein kinase 2 antibody
    EIF2AK1 antibody
    EIF2AK2 antibody
    Eukaryotic translation initiation factor 2 alpha kinase 2 antibody
    Eukaryotic translation initiation factor 2-alpha kinase 2 antibody
    HGNC:9437 antibody
    Interferon induced double stranded RNA activated protein kinase antibody
    Interferon inducible elF2 alpha kinase antibody
    Interferon inducible RNA dependent protein kinase antibody
    Interferon-induced, double-stranded RNA-activated protein kinase antibody
    Interferon-inducible RNA-dependent protein kinase antibody
    MGC126524 antibody
    P1/eIF-2A protein kinase antibody
    P1/eIF2A protein kinase antibody
    p68 kinase antibody
    PKR antibody
    PPP1R83 antibody
    PRKR antibody
    Protein kinase interferon inducible double stranded RNA dependent antibody
    Protein kinase RNA activated antibody
    Protein kinase RNA-activated antibody
    Protein phosphatase 1 regulatory subunit 83 antibody
    Serine/threonine protein kinase TIK antibody
    Tyrosine protein kinase EIF2AK2 antibody
  • Properties

  • Form Liquid
  • Concentration 1 mg/mL.
  • Storage instructions Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
  • Buffer 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
  • Isotype IgG
  • Purity ProA affinity purified
  • Subcellular location Cytoplasm, Nucleus.
  • Database links P19525(Human)
  • Applications

    WB: 1:1,000-1:2,000
    IHC: 1:50-1:200

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Specific Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

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